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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP.
Qualifier:
according to
Guideline:
other: EEC Directive 79/831 Annex V, Part C: Methods for the determination of Ecotoxicity, 5.2. Biotic degradation
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Initial cell/biomass concentration: Colony forming unit per mL used for the test were 1.1x10³
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium:
- Test temperature: 22 °C
- Continuous darkness: yes
- Other: Reaction vessels were filled with 900 mL of nutrient solution, inoculated with 0,5 mL/L of the inoculum and coverd with aluminum foile assurinh that the exchange of air between the flask and the surrounding atmosphere was not unduly impeded.

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 2
- Measuring equipment:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency: day 0, 7, 14, 21 and 28

CONTROL AND BLANK SYSTEM
- Other: To exclude the decrease of DOC by volatilisation of the test substance, four tests vessels containing nutrient solution and nutrient solution with test substance (ca. 20 mg/L), respectively, but no inoculum, were incubated for 6 days. Growth of microorganisms was prevented by adding 1 mL HgCl2-solution.
Reference substance:
aniline
Parameter:
% degradation (DOC removal)
Value:
95.3
Sampling time:
28 d
Details on results:
A biodegradation about 90 % was observed within the time window of 10 days.
Results with reference substance:
The results are valid as the control chemical yielded a DOC-removal of >70 % within 10 days during the test duration of 28 days, counting from the day that the observed level of biodegradation first exceeded 10 %.

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable

Description of key information

Readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The biodegradation of 2-dimethylaminoethyl methacrylate was investigated in two different guideline studies. The first study was conducted according to “EEC Directive 79/831 Annex V, Part C: Methods for the determination of Ecotoxicity, 5.2. Biotic degradation” using domestic activated sludge, non-adapted. The result of this investigation showed 95.3% degradation after 28 days passing the 10-day window. A OECD 301E guideline study showed a biodegradation of >95% within 28 days testing sewage, predominantly domestic.