2-dimethylaminoethyl methacrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Original report is not available. However, the report is cited by a peer-reviewed OECD SIDS.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: orange killfish
- Source: Sankyo Lab Service Co., LTD. (Japan)
- Age at study initiation (mean and range, SD): not described
- Length at study initiation (length definition, mean, range and SD): 2.1 cm (2.0 - 2.3 cm)
- Weight at study initiation (mean and range, SD): 0.13 g (0.1 - 0.2 g)
- Feeding during test: no. Not fed 24 h before the test started.


ACCLIMATION
- Health during acclimation (any mortality observed): < 5%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

30.3 mg/L

Test temperature:

23 - 25 °C

pH:

7.22 - 7.61

Dissolved oxygen:

5.31 - 8.70 mg/L

Nominal and measured concentrations:

Test concentrations (nominal): 10, 18, 32, 56, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 3 L test solution in a 3 L glass beaker
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h light-darkness cycle

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

19.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% C.I. = 15.8 - 23.5 mg/L

Sublethal observations / clinical signs:

Measured concentration of MADAME during a 24 h exposure test condition:

Nominal conc. (mg/L)	Measured Conc. (mg/L)			Percent of nominal
	0 h	24 h	Mean
Control	N.D.	N.D.	N.D.
10	9.20	4.55	6.47	65
18	17.0	6.86	10.8	60
32	24.1	8.37	14.2	44
56	41.3	12.8	23.0	41
100	88.7	13.8	35.0	35

Cummulative mortality:

Nominal conc. (mg/L)	Cummulative number of dead
	0 h	24 h	72 h	96 h
Control	0	0	0	0
10	0	0	0	0
18	0	0	1	1
32	0	0	1	2
56	3	3	4	6
100	10	10	10	10

Description of key information

Acute harmful for fish.

Additional information

For the assessment of the acute toxicity to fish of the test substance three studies are reported using three different fresh water species. The results indicates that 2-dimethylaminoethyl methacrylate is acute harmful to fish. For instance, in a GLP OECD guideline study with Oryzias latipes as test organism a LC50 (96 h) of 19.1 mg/L was determined using a semi-static test system. In contrast, no acute toxicity was determined in a GLP DIN 38 412 L15 study with Leuciscus idus (LC50 (96 h) = 331 – 592 mg/L). Moreover, no acute toxicity was demonstrated after 72 h of exposure to goldfish (LC50 = 139.5 mg/L).