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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Original report is not available. However, the report is cited in a peer-reviewed OECD SIDS. Hence, the reliability was taken from the OECD repor
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: National Institute for Environmental Studies (Japan)
- Age of parental stock (mean and range, SD): < 24 h
- Feeding during test
- Food type: Chlorella vulgaris
- Amount: 0.18 mg carbon/day/inividual
- Frequency: daily
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
29.5 - 39.3 mg/L
Test temperature:
20.4 - 21.0 °C
pH:
7.63 - 7.74 (control)
8.70 - 8.83 (63.2 mg/L)
Dissolved oxygen:
7.56 - 8.70 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 80 mL test solution in a 100 mL glass beaker
- Renewal rate of test solution (frequency/flow rate): every 24 h


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h light-darkness cycle
- Light intensity: < 1200 lux
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
4.35 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
7.86 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
16.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality

Measured concentrations:

Nominal conc. (mg/L)

Measured conc. (mg/L)

0 d

1 d

7 d

8 d

14 d

15 d

20 d

21 d

new

old

new

old

new

old

new

old

0.632

0.695

0.343

0.641

0.350

0.583

0.383

0.582

0.359

2.0

1.90

0.97

1.83

1.02

1.98

1.14

1.94

1.09

6.32

6.14

2.79

5.96

2.92

6.08

3.16

6.15

3.04

20.0

14.6

6.07

16.1

7.07

18.6

7.31

18.3

7.15

63.2

60.0

16.9

58.3

17.2

-

-

-

-

Cumulative number of juveniles produced per adult alive for 21 days.

 

Nominal conc. mg/L

 

(Measured conc. mg/L)

 

control

0.632

2.0

6.32

20.0

 

-

(0.479)

(1.44)

(4.35)

(11.2)

Mean (n=10)

67.8

67.6

57.2

62.1

7.9

S.D. (n=10)

10.1

11.0

10.5

12.1

6.4

In the highest test concentration (63.2 mg/L nominal) all parental animals were dead after 21 days.

Validity criteria fulfilled:
yes

Description of key information

Key value for chemical safety assessment

Additional information

For 2-dimethylaminoethyl methacrylate, one GLP OECD guideline (211) study on long-term toxicity to aquatic invertebrates is reported. After 21 days of exposure a clear effect on the reproduction was measured (EC50 = 7.86 mg/L). The corresponding NOEC was determined at 4.35 mg/L.