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EC number: 216-721-0 | CAS number: 1653-19-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-04-20 till 2010-05-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline and GLP-compliant study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- To avoid evaporation of the test item, the Erlenmeyer flasks were closed tightly with glass stoppers. Therefore the pH value decreased more than 1.5 pH units. This minor deviation is not considered to have any impact on the putcome of the study.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,3-dichloro-1,3-butadiene
- IUPAC Name:
- 2,3-dichloro-1,3-butadiene
- Reference substance name:
- 2,3-dichlorobuta-1,3-diene
- EC Number:
- 216-721-0
- EC Name:
- 2,3-dichlorobuta-1,3-diene
- Cas Number:
- 1653-19-6
- Molecular formula:
- C4H4Cl2
- IUPAC Name:
- 2,3-dichlorobuta-1,3-diene
- Reference substance name:
- 2,3-dichlorobutadiene-1,3
- IUPAC Name:
- 2,3-dichlorobutadiene-1,3
- Details on test material:
- Partie number - Q101
Sample number - 1152
Purity - not stated
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken for analysis from each test treatment and the control at the start of the study and at 24 hour intervals until the end of the study.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution was prepared daily,271.2 uL of the test substance was added to 2 L of dilution water. Undissolved particles were removed by filtration through a 7 - 12 um pore size filter paper. The pH of the stock solution was 8.0. The test media were prepared by addition of appropriate volumes of the stock solution to test vessels and dilution to 100 mL with water. Three replicates were prepared for each test treatment and control. Test vessels were filled to reduce loss of test material due to volatisation.
A volume of 0.667 mL of algal inoculum was added to each replicate vessel resulting in a final volume of 5000 cells/mL.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Desmodesmus subspicatus (formerly Scenedesmus subspicatus) Strain No. 86.81 SAG
Strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen (Germany).
Exponentially-growing stock cultures were maintained in the test facility under constant temperature conditions (21-24°C with a maximum fluctuation of +/- 2°C) at a light intensity in the range 60 – 120 µE. x m-2 x s-1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The growth medium (according to BRINGMANN & KÜHN (1977) was renewed once a week. Cell density measurements were made using a microcell- counter, Sysmex F300, Digitana.
Pre-cultures were set up three days before the start of a test. They were grown under identical exposure conditions as the stock cultures, except from the use of a different growth medium
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- Temperature range was 21 - 24°C
- pH:
- See table in the results section for details.
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal exposure concentrations: 0 (control), 5.0, 10, 20, 40, 80 and 160 mg/L. Geometric mean measured concentrations were 0.422, 0.888, 1.464, 2.869, 5.855 and 17.182mg/L.
- Details on test conditions:
- At the average of the test solutions, a light intensity in the range 60 to 120 µE. x m-2 x s-1, or an equivalent range of 4000 to 8000 lx, was used. Light intensity was checked before start of the study.
Light chamber in which a temperature in the range 21°C to 24°C was maintained at +/- 2°C, and continuous uniform illumination was provided in the spectral range 400 to 700 nm. Temperature was measured and recorded daily in a water filled flask which was incubated under the same conditions as the test flasks. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 3.8 - 5.0
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1.7 - 2.9
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 0.422 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 2.7 - 3.2
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 1.3 - 1.9
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.422 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- All calculations were perfofmed using the statistical programme ToxRatPro Version 2.10 (relaeased 20.02.2010). EC50 values were calcuated by probit analysis. NOEC and LOEC values were determined by multisampling comparison, using Williams Multiple Sequential t-Test.
Any other information on results incl. tables
Measured 2,3-dichloro-1,3-butadiene concentrations
Test item |
GC values |
||||
0 h |
24 h |
48 h |
72 h |
Geometric mean |
|
Control |
< 0.629 |
< 0.629 |
< 0.629 |
< 0.629 |
- |
5.0 |
0.793 |
0.399* |
0.405* |
0.247* |
0.422 |
10 |
1.578 |
0.869 |
0.917 |
0.495* |
0.888 |
20 |
2.694 |
1.406 |
1.398 |
0.868 |
1.462 |
40 |
5.199 |
2.765 |
2.900 |
1.625 |
2.869 |
80 |
10.562 |
5.540 |
5.872 |
3.420 |
5.855 |
160 |
29.598 |
16.587 |
15.603 |
11.378 |
17.182 |
*Values below the limit of quantification (0.629 mg/L) were calculated on basis of the mean recovery rate of the remaining concentrations at 24, 48 and 72 hour
pH values at start and end of the study
Test item |
pH values at 0 h and 72 h |
|
0 h |
72 h |
|
Control |
7.5 |
10.4 |
5.0 |
7.7 |
10.5 |
10 |
7.8 |
10.5 |
20 |
7.8 |
10.5 |
40 |
7.8 |
9.4 |
80 |
7.8 |
8.0 |
160 |
7.8 |
7.9 |
Cell numbers during the study
Mean measured concentrations (mg/L) |
Mean cell number (initial value 0.5 X 104cells/mL) |
||
24 hours |
48 hours |
72 hours |
|
Control |
2.0 |
7.7 |
20.0 |
0.422 |
2.0 |
8.0 |
19.0 |
0.888 |
2.0 |
7.7 |
18.0 |
1.464 |
1.7 |
6.3 |
18.7 |
2.869 |
1.0 |
3.0 |
10.7 |
5.855 |
1.0 |
1.0 |
1.3 |
17.182 |
1.0 |
1.0 |
1.0 |
Effects over the 72 hour exposure period:
Mean measured concentrations (mg/L) |
72 h growth rate
|
% inhibition compared to the control |
72 h yield
|
% inhibition compared to the control |
Control |
1.229 ±0.03 |
- |
19.5 ± 2.0 |
- |
0.422 |
1.212 ±0.02 |
1.3 |
18.5 ± 1.0 |
5.1 |
0.888 |
1.195 ±0 |
2.8 |
17.5 ± 0 |
10 |
1.464 |
1.206 ±0.03 |
1.8 |
18.2 ±1.5 |
6.8 |
2.869 |
1.202 ± 0.02 |
17 |
10.2 ± 0.58 |
48 |
5.855 |
0.308 ± 0.13 |
75 |
0.83 ± 0.58 |
96 |
17.182 |
0.231 ±0 |
81 |
0.5 ± 0 |
97 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on mean measured concentrations the 72 hour ErC50 value for Scenedesmus subspicatus exposed to 2,3-dichlorobutadiene-1,3 was calculated to be 4.4 mg/L (with 95% confidence intervals of 3.8 - 5.0 mg/L), the corresponding no-observed effect concentration (NOEC) was 0.888 mg/L. The 72 hour EyC50 value was calculated to be 2.9 mg/L (with 95% confidence intervals of 2.7 – 3.2 mg/L), the corresponding NOEC was 0.422 mg/L.
- Executive summary:
The alga Scenedesmus subspicatus were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 5.0, 10, 20, 40, 80 and 160 mg/L for 72 hours according to EU Method C.3 Freshwater Alga and Cyanobacteria, Growth Inhibition test (2009). To reduce volatilisation of 2,3-dichlorobutadiene-1,3 the study was conducted in sealed test vessels.
Geometric mean measured concentrations were 0.422, 0.888, 1.464, 2.869, 5.855 and 17.182 mg/L in the nominal 5.0, 10, 20, 40, 80 and 160 mg/L treatments. Based on mean measured concentrations the 72 hour ErC50value was calculated to be 4.4 mg/L (with 95% confidence intervals of 3.8 - 5.0 mg/L), the corresponding no-observed effect concentration (NOEC) was0.888 mg/L. The 72 hour EyC50value was calculated to be 2.9 mg/L (with 95% confidence intervals of 2.7 – 3.2 mg/L), the corresponding NOEC was 0.422 mg/L.
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