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EC number: 216-721-0
CAS number: 1653-19-6
The test substance did not induce gene mutations at the HPRT locus in
Chinese Hamster V79 cells in vitro, and is therefore considered to be
non-mutagenic under the conditions of the study.
The mutagenic potential of 2,3 -dichloro-1,3 -butadiene was determined
in Chinese hamster V79 cells by investigating gene mutations at the HPRT
An initial cytoxicity assay was performed to determine dose levels for
the main study. In the main study, two independent experiments were
performed each using two parallel cultures. The first mutagenicity
experiment was performed with and without metabolic activation (S9 mix)
and a treatment period of 4 hours. The second mutagenicity experiment
was performed with a treatment time of 4 hours with metabolic
activation, and 24 hours without. The highest concentration tested was
No substantial and reproducible dose dependent increase of the mutation
frequency was observed in either mutagenicity experiment. Positive
controls induced a distinct increase in mutant colonies.
In conclusion, 2,3 -dichloro-1,3 -butadiene did not induce gene
mutations at the HPRT locus in V79 cells under the conditions of the
study and therefore is considered to be non-mutagenic in this assay.
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