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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010-04-19 till 2010-05-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline and GLP-compliant study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
Partie number - Q101
Sample number - 1152
Purity - not stated
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples were taken from each of the test media at the start (0 hours), 24 hours and end of the exposure (48 hours). A sample was taken from the control at 0 and 48 hours.

Test solutions

Vehicle:
no
Details on test solutions:
A stock solution was prepared daily, 102 uL of the test substance was added to 1 L of dilution water and stirred for 10 minutes with a magnetic stirrer.Following stirring any undissolved particles were removed by filtration through a 7 - 12 um pore size filter paper. The pH of the stock solution was adjusted to 8.0. The test media were prepared by addition of appropriate volumes of the stock solution to a volume of 1000 mL. Two replicates were prepared for each test treatment, containing 250 - 300 mL each containing 10 Daphnia.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia were obtained from the laboratory own's culture by parthenogenic reproduction. The source of the Daphnia was the Bundesgesundheitsamt, Berlin, Germany. A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
Hardness of the M4 medium as 271 mg/L as CaCO3.
Test temperature:
Temperature range during the study was 20.2 - 21.1 °C
pH:
The pH range during the study was 8.0 - 8.1
Dissolved oxygen:
Dissolved oxygen (as % air saturation value) range during the study was 92 - 97%
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal exposure concentrations were 0 (control), 0.63, 1.25, 2.5, 5.0 and 10 mg/L. The geometric means obtained from the study were 0.251, 0.59, 1.21, 2.42 and 4.38 mg/L in the nominal 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments.
Details on test conditions:
The test vessels were BOD bottles, containing 250 - 300 mL of test media. Ten Daphnia were added to each vessel which was then sealed with a glass stopper. The study was performed with a 16 hour light and 8 hour dark cycle.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2.23 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.305 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5.47 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The results of the study are presented in tabular form below.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Probit analysis: Selected effective concentrations (ECx) of the test item and their 95% confidence limits (according to Fieller`s theorem).

Any other information on results incl. tables

Measured 2,3-dichlorobutadiene-1,3 concentrations.

Date

Time

Test item Concentration (mg/l)

2010

0.63

1.25

2.5

5

10

20. Apr

0 h

0.338

0.672

1.394

2.787

4.968

 

 

 

 

 

22. Apr

48 h

0.251

0.497

1.021

2.043

3.764

 

 

 

 

 

 

Geometric mean values for 2,3-dichlorobutadiene-1,3

Results:

 

0.29

0.58

1.19

2.39

4.32

geometric mean

 

 

 

 

 

 The test concentrations 0.63 and 1.25 were calculated on the basis of the mean recovery of the test concentrations 2.5, 5 and 10 mg/L.

Results of Daphnia study with 2,3-dichlorobutadiene-1,3

Mean measured concentration (mg/L)

Daphnia magna immobilisation

24 hours

48 hours

No. of Daphniaimmobilised

%

No. of Daphniaimmobilised

%

Control

0

0

0

0

0.29

0

0

0

0

0.58

0

0

0

0

1.19

0

0

5

25

2.39

15

75

20

100

4.32

20

100

20

100

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on mean measured concentrations of 2,3-dichlorobutadiene-1,3 the 24 hour EC50 value to Daphnia magna was calculated to be 2.22 mg/L and the 48 hour EC50 value was 1.28 mg/L. The no-observed effect concentration (NOEC) was observed to be 0.58 mg/L.
Executive summary:

Daphnia magna were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 0.63, 1.25, 2.5, 5.0 and 10 mg/L for 48 hours under static conditions according to EU Method C.2 Acute toxicity for Daphnia (2008).  Geometric mean measured concentrations were 0.29, 0.58, 1.19, 2.39 and 4.32 mg/L in the nominal 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments. Based on mean measured concentrations the 24 hour EC50value was calculated to be 2.22 mg/L and the 48 hour EC50value was 1.28 mg/L. The no-observed effect concentration (NOEC) was observed to be 0.58 mg/L.