2,3-dichlorobuta-1,3-diene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-04-19 till 2010-05-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline and GLP-compliant study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dichloro-1,3-butadiene

IUPAC Name:

2,3-dichloro-1,3-butadiene

Constituent 2

Reference substance name:

2,3-dichlorobuta-1,3-diene

EC Number:

216-721-0

EC Name:

2,3-dichlorobuta-1,3-diene

Cas Number:

1653-19-6

Molecular formula:

C4H4Cl2

IUPAC Name:

2,3-dichlorobuta-1,3-diene

Constituent 3

Reference substance name:

2,3-dichlorobutadiene-1,3

IUPAC Name:

2,3-dichlorobutadiene-1,3

Details on test material:

Partie number - Q101
Sample number - 1152
Purity - not stated

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken from each of the test media at the start (0 hours), 24 hours and end of the exposure (48 hours). A sample was taken from the control at 0 and 48 hours.

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution was prepared daily, 102 uL of the test substance was added to 1 L of dilution water and stirred for 10 minutes with a magnetic stirrer.Following stirring any undissolved particles were removed by filtration through a 7 - 12 um pore size filter paper. The pH of the stock solution was adjusted to 8.0. The test media were prepared by addition of appropriate volumes of the stock solution to a volume of 1000 mL. Two replicates were prepared for each test treatment, containing 250 - 300 mL each containing 10 Daphnia.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia were obtained from the laboratory own's culture by parthenogenic reproduction. The source of the Daphnia was the Bundesgesundheitsamt, Berlin, Germany. A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Hardness of the M4 medium as 271 mg/L as CaCO3.

Test temperature:

Temperature range during the study was 20.2 - 21.1 °C

pH:

The pH range during the study was 8.0 - 8.1

Dissolved oxygen:

Dissolved oxygen (as % air saturation value) range during the study was 92 - 97%

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal exposure concentrations were 0 (control), 0.63, 1.25, 2.5, 5.0 and 10 mg/L. The geometric means obtained from the study were 0.251, 0.59, 1.21, 2.42 and 4.38 mg/L in the nominal 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments.

Details on test conditions:

The test vessels were BOD bottles, containing 250 - 300 mL of test media. Ten Daphnia were added to each vessel which was then sealed with a glass stopper. The study was performed with a 16 hour light and 8 hour dark cycle.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

2.23 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.305 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

5.47 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The results of the study are presented in tabular form below.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Probit analysis: Selected effective concentrations (ECx) of the test item and their 95% confidence limits (according to Fieller`s theorem).

Any other information on results incl. tables

Measured 2,3-dichlorobutadiene-1,3 concentrations.

Date	Time	Test item Concentration (mg/l)
2010		0.63	1.25	2.5	5	10
20. Apr	0 h	0.338	0.672	1.394	2.787	4.968
22. Apr	48 h	0.251	0.497	1.021	2.043	3.764

 

Geometric mean values for 2,3-dichlorobutadiene-1,3

Results:		0.29	0.58	1.19	2.39	4.32
geometric mean

 The test concentrations 0.63 and 1.25 were calculated on the basis of the mean recovery of the test concentrations 2.5, 5 and 10 mg/L.

Results of Daphnia study with 2,3-dichlorobutadiene-1,3

Mean measured concentration (mg/L)	Daphnia magna immobilisation
	24 hours		48 hours
	No. of Daphniaimmobilised	%	No. of Daphniaimmobilised	%
Control	0	0	0	0
0.29	0	0	0	0
0.58	0	0	0	0
1.19	0	0	5	25
2.39	15	75	20	100
4.32	20	100	20	100

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on mean measured concentrations of 2,3-dichlorobutadiene-1,3 the 24 hour EC50 value to Daphnia magna was calculated to be 2.22 mg/L and the 48 hour EC50 value was 1.28 mg/L. The no-observed effect concentration (NOEC) was observed to be 0.58 mg/L.

Executive summary:

Daphnia magna were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 0.63, 1.25, 2.5, 5.0 and 10 mg/L for 48 hours under static conditions according to EU Method C.2 Acute toxicity for Daphnia (2008).  Geometric mean measured concentrations were 0.29, 0.58, 1.19, 2.39 and 4.32 mg/L in the nominal 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments. Based on mean measured concentrations the 24 hour EC₅₀value was calculated to be 2.22 mg/L and the 48 hour EC₅₀value was 1.28 mg/L. The no-observed effect concentration (NOEC) was observed to be 0.58 mg/L.