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Zebra fish were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 9.4, 20.7, 45.5 and 100 mg/L for 96 hours under semi-static conditions according to EU Method C.1 Acute toxicity to fish (2008). Analysis of new and 24 hour aged media was performed for each treatment and geometric mean values were calculated for each 24 hour exposure period. The 96 hr LC50based on the geometric mean measured concentrations was 1.39 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 0.501 mg/L (Neuhahn, 2010).

Zebra fish were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 1, 3.2, 10 and 31.6 mg/L for 96 hours under static conditions according to Letale Wirkung beim Zebrabarbling - Brachydanio rerio (LC0, LC 50, LC100: 48 - 96 Stunden) Verfahrensvorschlag: umweltbundesamt Berlin, Stand Mai 1984 guideline. No analysis of the test media was performed. The 96 hr LC0 based on the nominal concentrations was 3.2 mg/L and the 96 h LC100was 31.6 mg/L (Kanne, 1991).

Daphnia magna were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 0.63, 1.25, 2.5, 5.0 and 10 mg/L for 48 hours under static conditions according to EU Method C.2 Acute toxicity for Daphnia (2008).  Geometric mean measured concentrations were 0.29, 0.58, 1.19, 2.39 and 4.32 mg/L in the nominal 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments. Based on mean measured concentrations the 24 hour EC50value was calculated to be 2.22 mg/L and the 48 hour EC50value was 1.28 mg/L. The no-observed effect concentration (NOEC) was observed to be 0.58 mg/L (Neuhahn, 2010).

Daphnia magna were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 1.6, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L for 48 hours under static conditions according to EU Method C.2 Acute toxicity for Daphnia.  Based on mean measured concentrations the 48 hour EC50value was calculated to be 1.502 mg/L (with 95% confidence intervals of 0.849 to 3.483 mg/L). The no-observed effect concentration (NOEC) was observed to be 0.839 mg/L (Bruns, 2005).

The alga Scenedesmus subspicatus was exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 5.0, 10, 20, 40, 80 and 160 mg/L for 72 hours according to EU Method C.3 Freshwater Alga and Cyanobacteria, Growth Inhibition test (2009).  To reduce volatilisation of 2,3-dichlorobutadiene-1,3 the study was conducted in sealed test vessels.

Geometric mean measured concentrations were 0.422, 0.888, 1.464, 2.869, 5.855 and 17.182 mg/L in the nominal 5.0, 10, 20, 40, 80 and 160 mg/L treatments. Based on mean measured concentrations the 72 hour ErC50value was calculated to be 4.4 mg/L (with 95% confidence intervals of 3.8 - 5.0 mg/L), the corresponding no-observed effect concentration (NOEC) was 0.888 mg/L. The 72 hour EyC50value was calculated to be 2.9 mg/L (with 95% confidence intervals of 2.7 – 3.2 mg/L), the corresponding NOEC was0.422 mg/L (Neuhahn, 2010). 

The alga Scenedesmus subspicatus was exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control) and 100 mg/L for 72 hours according to EU Method C.3 Freshwater Alga and Cyanobacteria, Growth Inhibition test. Measured concentrations were determined in vessels sealed with either glass stoppers or cotton. Men measured concentrations were >3.12 mg/L using glass stoppers or >2.76 mg/L using cotton stoppers. Both the 72 h EbC50and ErC50values were determined to be >100 mg/L based on nominal exposure concentrations. The corresponding 72 h EbC50or ErC50values were either >3.16 or >2.76 mg/L depending on the analytical values used (Bruns, 2005).  

The toxicity of 2,3-dichlorobutadiene-1,3 to activated sewage sludge was investigated in a respiration inhibition test according to EU Method C11 Activated sludge respiration inhibition 2008. Following three hours exposure to 2,3-dichlorobutadiene-1,3 the EC50was observed to be > 1000 mg/L (based on nominal exposure concentrations), the corresponding EC10was calculated to be 163.8 mg/L (Weyers, 2010).