Acetonitrile

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

Effects were evaluated following repeated topical exposure

GLP compliance:

not specified

Test organisms (species):

Lumbricus sp.

Animal group:

nematods

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Age at test initiation: sexually mature
- Weight at test initiation: 4-5 g

ACCLIMATION
- Acclimation period: one week
- Acclimation conditions: held in a medium consisting of nonsterilized potting soil fortified with cornmeal at 13°C

Study type:

laboratory study

Substrate type:

artificial soil

Post exposure observation period:

21 days.

Test temperature:

13 degree C after treatment occurred.

Details on test conditions:

TEST SYSTEM
- Topical application: Instrumentation Specialties microapplicator with a 1.0-ml Hamilton Syringe fitted with a 27-gauge needle.
- Volume: 1-4 ul
- Area of application: dorsum, anterior to the clitellum.

OTHER TEST CONDITIONS
- Light intensity: dark

EFFECT PARAMETERS MEASURED: criteria for a toxic response included weight loss, appearance of sores or swellings, coiling, and mortality.

Duration:

21 d

Dose descriptor:

other: Percentage mortality

Effect conc.:

156 - 780 other: mg/kg (applied topically to earthworms)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

Acetonitrile caused no irritation or mortality when applied topically to earthworms at doses estimated to be in the range of 156 mg/kg to 780 mg/kg over 21 days.

Toxicity of DDT to the Earthworm via Topical Application in Acetonitrile

		% Mortality	% Mortality	% Mortality	% Mortality
Dose	Solvent	24 h	7 d	14 d	21 d
200	Acetonitrile	10	20	20	20
100	Acetonitrile	10	20	20	20
50	Acetonitrile	0	0	0	0
25	Acetonitrile	10	20	20	20
5	Acetonitrile	0	10	10	10
0	Acetonitrile	0	0	0	0

Conclusions:

Scrutiny of several different solvents revealed that acetonitrile caused no irritation when applied topically to the earthworm

Executive summary:

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated with acetonitrile by topical application. Acetonitrile was a solvent control used in this study. Doses of 1 -4 ul were applied topically to worms weighing 4 -5 g. This corresponds to doses in the range of 156 mg/kg to 780 mg/kg.

Description of key information

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated by topical application to acetonitrile doses estimated to be in the range of 156 mg/kg to 780 mg/kg.

Additional information

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated with acetonitrile by topical application. Acetonitrile was a solvent control used in this study. Doses of 1 -4 ul were applied topically to worms weighing 4 -5 g. This corresponds to doses in the range of 156 mg/kg to 780 mg/kg.