Registration Dossier

Administrative data

Endpoint:
repeated dose toxicity: dermal
Remarks:
other: repeated dose
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original report in German was not fully translated. Only summary results are presented.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1960
Report Date:
1960

Materials and methods

Principles of method if other than guideline:
Repeated application of acetonitrile to rabbit ears.
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Acetonitrile
- Analytical purity: 99.5% according to manufacturer

Test animals

Species:
rabbit
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
not specified
Details on exposure:
Repeated application to both ears.
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
24 hrs for each treatment
Frequency of treatment:
4 treatments over ten days.
Doses / concentrations
Remarks:
Doses / Concentrations:
4 ml (2 ml on each ear)
Basis:

No. of animals per sex per dose:
3 rabbits

Results and discussion

Effect levels

Basis for effect level:
other: light purple skin discoloration but no symptoms of systemic poisoning
Remarks on result:
not measured/tested
Remarks:
Effect level not specified (migrated information)

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

The repeated application of 4 ml acetonitrile per animal on both ears resulted in light purple skin discoloration. Systemic poisoning symptoms were not observed. Investigation of the urine revealed no pathological findings. Body weights decreased during the study period, but recovered during a three week follow-up period.

Applicant's summary and conclusion

Executive summary:

BASF (1960) reported light purple skin discoloration but no symptoms of systemic poisoning following repeated (4 treatments) application of acetonitrile to the ears of rabbits.