2-{2-chloro-4-mesyl-3-[(tetrahydrofuran-2-ylmethoxy)methyl]benzoyl}cyclohexane-1,3-dione

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 Nov - 10 Dec 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: “Test on Hydrolyzability” of agricultural chemicals stipulated in the “Notification Concerning Test Data for Registration of Agricultural Chemicals” (12 Nohsan No. 8147)

Version / remarks:

November 24, 2000, revised on December 10, 2002

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: The test solution was quantitatively analyzed at each measurement point
- Sampling intervals/times for pH measurements: Days 0 and 5

Buffers:

pH 4: 2 ml of 0.1 N sodium hydroxide was added to 250 mL of 0.1 M potassium hydrogen phthalate and the volume was adjusted to 500 mL with purified water.
pH 7: 148 mL of 0.1 N sodium hydroxide was added to 250 mL of 0.1 M potassium dihydrogen phosphate and the volume was adjusted to 500 mL with purified water.
pH 9: 106 mL of 0.1 N sodium hydroxide was added to 250 mL of 0.1M boric acid and the volume was adjusted to 500 mL with purified water.

Details on test conditions:

TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: Glass-stoppered Erlenmeyer flask (100 mL capacity, glass)
- Sterilisation method: use of an autoclave
- Measures to exclude oxygen: Nitrogen gas was bubbled through each buffer solution for 5 minutes to expel dissolved oxygen
TEST MEDIUM
- Volume used/treatment: 100 mL
- Preparation of test medium: The test substance stock solution (2.50 mL) was withdrawn and diluted with the buffer solution of desired pH to prepare the test solution (250 mL)

Duration:

5 d

pH:

4

Temp.:

50 °C

Initial conc. measured:

100 mg/L

Duration:

5 d

pH:

7

Temp.:

50 °C

Initial conc. measured:

100 mg/L

Duration:

5 d

pH:

9

Temp.:

50 °C

Initial conc. measured:

100 mg/L

Number of replicates:

2

Positive controls:

no

Negative controls:

no

Transformation products:

not measured

% Recovery:

94.3

pH:

4

Temp.:

50 °C

Duration:

5 d

pH:

7

Temp.:

50 °C

Duration:

5 d

Remarks on result:

other:

Remarks:

Total recovery of test substance: 101%

% Recovery:

100

pH:

9

Temp.:

50 °C

Duration:

5 d

Key result

pH:

4

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Key result

pH:

7

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Key result

pH:

9

Temp.:

25 °C

DT50:

> 1 yr

Type:

not specified

Details on results:

TEST CONDITIONS
- pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes/

Description of key information

The test substance was shown to be hydrolytically stable at pH 4, 7, and 9 (DT50: > 1 year at 25 °C).

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C

Additional information

The available study, following GLP and OECD Guideline 111, assessed the hydrolytic degradation of the test substance at 25 °C and under alkaline (pH 9), neutral (pH 7) and acidic (pH 4) conditions. A preliminary hydrolysis test was performed at 50 °C in aqueous solution at pH 4, 7 and 9. The mean residual rate of the test substance was at least 90% at all pH levels under the test conditions used in the present study (50 °C, 5 days). Therefore, the test substance was shown to be hydrolytically stable (DT50 (25 °C) > 1 year). On the basis of the results from the preliminary test, no further work was conducted.