Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-514-3 | CAS number: 2451-62-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: not stricty according to OECD 471, no statistical analysis
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- not stricty according to OECD 471, no statistical analysis
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3,5-tris(oxiranylmethyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- EC Number:
- 219-514-3
- EC Name:
- 1,3,5-tris(oxiranylmethyl)-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- Cas Number:
- 2451-62-9
- Molecular formula:
- C12H15N3O6
- IUPAC Name:
- tris[(oxiran-2-yl)methyl]-1,3,5-triazinane-2,4,6-trione
- Details on test material:
- Araldite PT810 (TGIC), 1,3,5-triglycidylisocyanurate.
Purity: Commercial grade (> 97%)
Impurity < 100 ppm epichlorohydrin (CAS-no. 106-89-8)
Constituent 1
Method
- Target gene:
- histidine auxotrophe
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9- rat liver extract Arochlor 1254 induced
- Test concentrations with justification for top dose:
- 1.22 - 10000 microgram/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: 2-nitrofluorene, quinacrine mustard, 2-anthramine
- Details on test system and experimental conditions:
- Positive control substances were Sodium azide (in water) for nonactivated TA-1535 & TA-100; 2-nitrofluorene (in DMSO) for nonactivated TA-1538 & TA-98; Quinacrine mustard (in DMSO) for nonactivated TA-1537; 2-Anthramine (in DMSO) for all activated strains
All strains are histine auxotrophs; only revertant mutations to histidine independence are growing in medium with minimal amounts of histidine. Spontaneous reverse mutations are rare, and only mutation rates of 2-100 fold are considered positive. Strains were received from Bruce Ames.
Media used were: Oxoid Media #2 (nutrient broth) for overnight growth at 37°C; Selection media for mutagenicity assay was Vogel Bonner Medium E (Vogen and Bonner, 1956) with 2% glucose and 1.5% bactoagar.; overlay medium was 0.6% purified agar, 10% of 0.5 mM L-Histidine and 0.5 mM Biotin, and 0.5% NaCl according to Ames et al (1975).
Assay were performed with and without S9 activating homogenate.
S9 was 9000 xg supernatant from Sprague-Dawley adult male rat liver induced by Aroclor 1254. The S9 mix consited of NADP (sadium salt) 4 μmoles, D-Glucose-6-phosphate 5 μmoles, MgCl2 8 μmoles, KCl 33 μmoles, Na-Phosphate buffer pH 7.4 100 μmoles, and S9 homogenate 100 μlitres
Dose selection was performed with TA-100 in the non-activated system in overlay agar contailing TGIC (0.05 -0.1 ml of appropriate solution), 0.1-0.2 ml indicator organisms, and 0.5 ml 0.2M phosphate buffer pH 7.4. This solution (at 43-45°C) was added to minimal agar plates and incubated at 37°C for two days. Colonies were counted manually on the plates. Reduction of colony count was considered as toxicity.
Main assay conduct:
Non-activated: 2 ml Overlay agar, 0.05-0.1 ml TGIC solution , 0.2 ml indicator organism, and 0.5 ml 0.2M Phosphate buffer pH 7.4, was swirled gently and poured over a minimal agar plate and incubated for 48 hours. Colonies were counted manually on the plates.
Activated: Same as non-activated , but instead of 0.5 ml =.2M Phosphate buffer pH 7.4, 0.5 ml of S9 mix is added.
Negative controls were run in parallel (only overlay agar with test organisms).
Strains TA-98 and TA-100: at least three concentrations in a row have to be positive, and the response has to be at least 2-times the control value.
Control values were : TA-1535: 8-30; TA-1537: 4-30; TA-1538: 10-40; TA-98: 20-75; TA-100: 80-250.
Experiments were performed in triplicates, and 2 independent experiments were performed. - Evaluation criteria:
- Evaluation of data: Strains TA-1535, TA-1537, TA-1538: at least three concentrations in a row have to be positive, and the response has to be at least 3-times the control value.
- Statistics:
- No statistical analysis of the data was performed.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
The assay was positive (mutagenic activity associated with TGIC, araldite PT 810).
Doses assayed were from 1.22 to 10’000 μg/plate, showing some toxicity 5000 and complete toxicity at 10’000 μg/plate with TA-100 in the preliminary assay.
Therefore, doses of 1-10’000 μg/plate were used throughout the experiment with all strains.
The positive controls were within the expected range of induced colonies
Applicant's summary and conclusion
- Conclusions:
- TGIC caused mutagenic effects in all strains tested with the exception of TA 1538 (ambiguous results) in the Ames test, and thus, it is mutagenic in-vitro
- Executive summary:
Araldite PT 810 (TGIC) was mutagenic in three different Salmonella strains (TA-1535, TA-98 and TA-100) in the presence and absence of S9-activating enzymes of rat liver induced with Aroclor 1254.Tehrefore, Araldite PT 810 is mutagenic in bacteria in-vitro. Based on the results of the non-activating system, it is obvious that TGIC is a Direct-Acting Mutagen
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
