Acetonitrile

Toxicity to aquatic plants other than algae

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

No information available.

GLP compliance:

not specified

Test organisms (species):

Lemna minor

Details on test organisms:

TEST ORGANISM
- Common name: Duckweed
- L. minor was cultured at 25°C in an aquarium using the culture solution recommended by the US EPA (US EPA, 1989) under continuous illumination of 11000 Lux light from an overhead fluorescent lamp. The culture condition was non-axenic. L. minor fronds of similar size and shape were selected for exposure.

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

27 +2°C

Nominal and measured concentrations:

0, 1000, 1800, 3200, 5600 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: culture dishes (9 cm diameter)
- Type: closed
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10 fronds
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- After 96 h exposure all the fronds in one container were put into a glass grinder containing 4ml 90% acetone (10% distilled water). The homogenate was transferred into one centrifuge tube of 10 ml after being grounded for 5 min, then 1 ml 90% acetone was added, and the solution was refrigerated for 72 h at 4°C to extract chlorophyll A. The homogenate was then centrifuged at 2000 rev min- 1 for 10 min. The optical density (00) values of the supernatant at 663 nm, 645 nm, 630 nm and 750 nm, respectively, were measured using a quartz glass curette with 1 cm thickness by a spectrophotometer (model 721, Shanghai Science Instrument Co., Shanghai, China).


OTHER TEST CONDITIONS
- Light intensity and quality: continuous illumination of 11000 Lux light from an overhead fluorescent lamp.

EFFECT PARAMETERS MEASURED: Growth inhibition
- Chlorophyll measurement: The chlorophyll A amount in every test container was indicated by the concentration of chlorophyll A in supernatant (Ca). Ca was calculated following Zhou (1990)

Duration:

96 h

Dose descriptor:

IC50

Effect conc.:

3 663 mg/L

Nominal / measured:

nominal

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

other: Chlorophyll a concentration

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Executive summary:

Tong and Hongjun (1997) reported a 96 -hour IC50 value of 3663 mg/L for acetonitrile in Duckweed, (Lemna minor).

Description of key information

Tong and Hongjun (1997) reported a 96 -hour IC50 value of 3663 mg/L for acetonitrile in Lemna minor (Duckweed).

Key value for chemical safety assessment

EC50 for freshwater plants:

3 663 mg/L

EC10 or NOEC for freshwater plants:

1 000 mg/L

Additional information

Tong and Hongjun (1997) reported a 96 -hour IC50 value of 3663 mg/L and a NOEC of 1000 mg/L for acetonitrile in Lemna minor (Duckweed).