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Toxicological information

Repeated dose toxicity: inhalation

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Administrative data

Endpoint:
sub-chronic toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1977
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Remarks:
Original reference in foreign language (Russian)

Data source

Reference
Reference Type:
publication
Title:
Characteristics of the response of animals of different ages to the single and chronic effect of benzonitrile
Author:
Agaev, F. B.; Alekperov, I. I.; Pavlova, L. P.
Year:
1977
Bibliographic source:
Gig. Tr. Prof. Zabol. (3), 40-2

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
exposure to 0.07 (+/- 0.01) mg/l air for 4 h/d (5 d/week; 4.5 month) in a 750 l chamber; dynamic method.
GLP compliance:
not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Test material form:
liquid

Test animals

Species:
rat
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
whole body
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
4 hours/day
Frequency of treatment:
5 days/week; 4.5 month
Doses / concentrations
Dose / conc.:
70 mg/m³ air (nominal)
Remarks:
Basis: nominal conc.
No. of animals per sex per dose:
10 per age (young/adult/old)
Control animals:
yes

Results and discussion

Effect levels

Dose descriptor:
conc. level: 70 mg/m³
Effect level:
70 mg/m³ air (nominal)
Based on:
test mat.
Sex:
not specified
Basis for effect level:
other: decrease of haemoglobin

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

First transient effects in adult rats occur after 5 days, normalizing until the end of the first month (except some CNS effects). After 90 days there was a significant decrease in the number of red blood cells in adults, together with an decrease in haemoglobin and increase in the number of leucocytes.

Applicant's summary and conclusion

Conclusions:
Subchronic exposure to Benzonitrile causes effects like a significant decrease in the number of red blood cells, decrease in haemoglobin and increase in the number of leucocytes. In contrast to acute exposure no age-dependency was found at a concentration of 0.07 mg/l in this study.
Executive summary:

In an inhalation toxicity study groups of 10 male albino rats of different ages (young, adult and old: body weight ranging from 60 to 370 g) were exposed to benzonitrile for 4 h in a 750 l chamber 5 days/week for 4.5 months at a concentrations of 0.07 mg/l air.

A significant decrease in the number of red blood cells, a decrease in haemoglobin and an increase in the number of leucocytes were observed. In contrast to acute exposure no age-dependency was found in this study.

Due to the poor documentation it is not possible to assess the relevance and reliability of this study.