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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1961
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficent for assessment.
Qualifier:
according to
Guideline:
other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
Principles of method if other than guideline:
Laboratory operations described in detail by Henderson and Tarzwell
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Lepomis macrochirus
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Duration:
24 h
Dose descriptor:
other: TLm
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Duration:
48 h
Dose descriptor:
other: TLm
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Key result
Duration:
96 h
Dose descriptor:
other: TLm
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water

Results for different bioassays use

and experimental conditions :

 

 

TLM / LC50 (mg/l)

Kind of Fish

Dilution Water

24 hour

48 hour

96 days

Bluegills

Soft

78.

78.

78.

Validity criteria fulfilled:
not applicable
Conclusions:
he 96-h TLm (median toxic level) of Benzonitrile for bluegill is 78 mg/L in soft water.
Executive summary:

The toxicity of benzonitrile for bluegill was tested under static condictions for groups of ten fish each at concentrations of

10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under soft water conditions.

The 96-h TLm (median toxic level) of Benzonitrile for bluegill was determined to be 78 mg/L in soft water.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1961
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment.
Qualifier:
according to
Guideline:
other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
Principles of method if other than guideline:
Laboratory operations described in detail by Henderson and Tarzwell
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Pimephales promelas
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Duration:
24 h
Dose descriptor:
other: TLm
Effect conc.:
116 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: hard water
Duration:
24 h
Dose descriptor:
other: TLm
Effect conc.:
240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Duration:
48 h
Dose descriptor:
other: TLm
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: hard water
Duration:
48 h
Dose descriptor:
other: TLm
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Key result
Duration:
96 h
Dose descriptor:
other: TLm
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: hard water
Duration:
96 h
Dose descriptor:
other: TLm
Effect conc.:
135 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water

Results for different bioassays use

and experimental conditions :

 

 

TLM / LC50 (mg/l)

Kind of Fish

Dilution Water

24 hour

48 hour

96 days

Fathead minnow

Hard

116.

78.

78.

Fathead minnow

Soft

240.

180.

135.

Validity criteria fulfilled:
not applicable
Conclusions:
The 96-h TLm (median toxic level) of Benzonitrile for fathead minnow is 78 mg/L in hard water and 135 mg/L in soft water.
Executive summary:

The toxicity of benzonitrile for fathead minnow was tested under static condictions for groups of ten fish each at concentrations of

10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under hard water conditions as well as under soft water conditions.

The 96-h TLm (median toxic level) of Benzonitrile for fathead minnow was determined to be 78 mg/L in hard water and 135 mg/L in soft water.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1961
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficent for assessment.
Qualifier:
according to
Guideline:
other: Method recommended by the subcommittee on Toxicity, Federation of Sewage and Industrial Wastes Association
Principles of method if other than guideline:
Laboratory operations described in detail by Henderson and Tarzwell
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Poecilia reticulata
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Duration:
24 h
Dose descriptor:
other: TLm
Effect conc.:
400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Duration:
48 h
Dose descriptor:
other: TLm
Effect conc.:
400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water
Key result
Duration:
96 h
Dose descriptor:
other: TLm
Effect conc.:
400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: soft water

Results for different bioassays use

and experimental conditions :

 

 

TLM / LC50 (mg/l)

Kind of Fish

Dilution Water

24 hour

48 hour

96 days

Guppies

Soft

400.

400.

400.

Validity criteria fulfilled:
not applicable
Conclusions:
he 96-h TLm (median toxic level) of Benzonitrile for guppy is 400 mg/L in soft water.
Executive summary:

The toxicity of benzonitrile for guppy was tested under static condictions for groups of ten fish each at concentrations of

10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The test was conducted under soft water conditions.

The 96-h TLm (median toxic level) of Benzonitrile for guppy was determined to be 400 mg/L in soft water.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1982
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Qualifier:
no guideline available
Principles of method if other than guideline:
no data; secondary reference
GLP compliance:
not specified
Remarks:
abstract from IFA GESTIS
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Brachydanio rerio (new name: Danio rerio) & Leuciscus idus
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
96 h
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
54 - 130 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Remarks on result:
other: median 92 mg/l; number of studies: 2
Validity criteria fulfilled:
not specified
Conclusions:
LC 50 (fish, 96 h) = 92 mg/l (median);
reported minimum value: LC50 = 54 mg/L
Executive summary:

A study conducted by Wellens, H. (1982: Comparison of the Sensitivity of Brachydanio rerio and Leuciscus idus by Testing the Fish Toxicity of Chemicals and Wastewaters. Z.Wasser-Abwasser-Forsch. 51(2):49-52.) is referenced in the GESTIS-database on hazardous substances (www.dguv.de/ifa/gestis-database). The reported median LC50 (96) for Brachydario rerio and Leuciscus idus is 92 mg/l. The LC50 ranges between 54 and 130 mg/L.

Description of key information

The reported range of the 96-h LC50 of benzonitrile for different fish species is between 54 mg/L and 135 mg/L. 

Key value for chemical safety assessment

LC50 for freshwater fish:
54 mg/L

Additional information

The toxicity of benzonitrile for fathead minnow, bluegill and guppy was tested under static conditions for groups of ten fish per concentration at levels of 10, 5.6, 3.2, 1.8 and 1.0 ppm for up to 96 hours. The tests were conducted under soft water conditions for all three fish species and in addition under hard water conditions for fathead minnow.

The 96-h TLm (median toxic level, equivalent to LC50) of benzonitrile for fathead minnow was determined to be 78 mg/L in hard water and 135 mg/L in soft water.

The 96-h TLm for bluegill and guppy was determined to be 78 mg/L and 400 mg/L, respectively, in soft water.

A study conducted by Wellens, 1982 is referenced in the GESTIS-database on hazardous substances (www.dguv.de/ifa/gestis-database). The reported median LC50 (96) for Brachydario rerio and Leuciscus idus is 92 mg/l. The minimum value determined in the study is 54 mg/L (species not reported).

As the value of 54 mg/L is the most sensitive value reported in all available studies, this value is used as key value for the chemical safety assessment.