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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report Date:
1980

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: 40 CFR Part 163.81-2
Principles of method if other than guideline:
EPA, Pesticides Programs, proposed guidelines for registering pesticides in the U.S., hazard evaluation: humans and domestic animals
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Duration of exposure:
24 hours
Doses:
2.0 g/kg, and thereafter 0.01, 0.05, 0.1, 0.5, and 1.0 g/kg,
No. of animals per sex per dose:
1 male + 1 female for range finding and 4 male + 4 female for LD50 determination

Control animals:
yes

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 400 mg/kg bw
Based on:
test mat.
95% CL:
1 - 1.8

Any other information on results incl. tables

Preliminary Range Finding Study:

Dose

Mortality

(g/kg)

 

0.01

0/2

0.05

0/2

0.1

0/2

0.5

0/2

1.0

0/2

 

Dose level, mortality data and LD50calculations:

 

Dose Level

Mortality

 

(g/kg)

Male

Female

Abraded Skin

Intact Skin

Total

Mortality

0.5

0/4

0/4

0/4

0/4

0/8

0.7

0/4

1/4

0/4

1/4

1/8

1.0

0/4

2/4

1/4

1/4

2/8

1.4

1/4

3/4

1/4

3/4

4/8

2.0

3/5

5/5

8/10

0/0

8/10

Control

(0/4)

(0/4)

(0/4)

(0/4)

(0/4)

LD50

 

1.8

1.0

1.55

1.05

1.4

95% Confidence Limit

 

1.2 to
2.4

0.6 to
1.4

1.15 to
1.95

0.65 to
1.45

1.0 to
1.8

 

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category IV
Conclusions:
The LD50 was calculated at 1.4 g/kg.
Executive summary:

In an acute dermal toxicity study, groups of 4 young adults New Zealand White rabbits/sex/dose were dermally exposed to Benzonitril (100 % a.i) for 24 hours to 10% of body surface area at doses of 0.5, 0.7, 1.0, 1.4 and 2.0 g/kg bw. Animals then were observed for 14 days.

 

Dermal LD50:

      Males =1.8 g/kg bw (95% C.I. 1.2 to 2.4 g/kg bw)

      Females = 1.0 g/kg bw (95% C.I. 0.6 to 1.4 g/kg bw.)

      Combined = 1.4 g/kg bw (95% C.I. 1.0 to 1.8 g/kg bw.)