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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report Date:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The test material was used to treat six rabbits each on the intact skin and the abraded skin.
Further details on the method are not available.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Type:
impurity
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
New Zealand White

Test system

Type of coverage:
occlusive
Preparation of test site:
other: abraded and intact skin
Vehicle:
unchanged (no vehicle)
Controls:
no
Duration of treatment / exposure:
24 h (0.5 ml)
Observation period:
first reading after the 24 h exposure, a second after 48 h (72 h after application)
Number of animals:
6 adults

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24 h
Score:
0.3
Reversibility:
fully reversible within: 7d
Remarks on result:
other: intakt skin
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 72 h
Score:
0.2
Reversibility:
fully reversible within: 7 d
Remarks on result:
other: intact skin
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24 h
Score:
1.3
Reversibility:
fully reversible within: 7 d
Remarks on result:
other: abraded skin
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 72 h
Score:
1
Reversibility:
fully reversible within: 7 d
Remarks on result:
other: abraded skin

Any other information on results incl. tables

Irritation was scored by the method of Draize (J.Pharacol.82 (1944) 377-390).

The second reading is carried out on the basis of the CIVO –grading system.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Remarks:
Criteria used for interpretation of results: other: CIVO grading system
Conclusions:
Benzonitrile is a slight skin irritant
Executive summary:

In a primary dermal irritation study 12 adult rabbits were exposed to 0,5 ml of benzonitrile for 24 hours to a 1 inch x 1 inch of body surface area. Animals then were observed for 2 days (48 hr). Irritation was scored by the method of Draize (J.Pharacol.82 (1944) 377- 390).

Since the Draize grading system does not comprise a 72h reading the second reading is carried out on the basis of the CIVO- grading system. Benzonitrile caused very slight erythema in two out of six rabbits treated on the intact skin. On the abraded skin it caused very slight or well- defined erythema in five out of six rabbits and very slight edema in one out of six rabbits. The average results suggest benzonitrile to be a very slight primary skin irritant.