Registration Dossier

Administrative data

Endpoint:
endocrine system modulation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientific publication with appropriate description of the method.

Data source

Reference
Reference Type:
publication
Title:
Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay.
Author:
Nishihara T, Nishikawa J, Kanayama T, Dakeyama F, Saito K, Imagawa M, Takatori S, Kitagawa Y, Hori S, Utsumi H
Year:
2000
Bibliographic source:
J. Health Sci., Vol. 46(4), 282-298, 2000

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The aim of this study was to assess the estrogenic activities of environmentally relevant chemicals including melamine.
Method according to Nishikawa et al. The yeast two-hybrid system is based on the ligand-dependent interaction of two proteins, a hormone receptor and a coactivator, and hormonal activity is detected by ß-galactosidase activity.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
melamine

Test animals

Species:
other: Saccharomyces cerevisiae Y 190

Administration / exposure

Route of administration:
other: in vitro assay
Duration of treatment / exposure:
Exposure period: 4 hour(s)
Details on study design:
Yeast Two-Hybrid Assay.

Results and discussion

Any other information on results incl. tables

Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.

Applicant's summary and conclusion

Conclusions:
Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.
Executive summary:

Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.