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EC number: 255-449-7
CAS number: 41583-09-9
Melaminephosphate was tested in the Salmonella
typhimurium reverse mutation assay with four histidine-requiring strains
of Salmonella typhimurium (TA1535, TA1537, TA100 and TA98) and in the
Escherichia coli reverse mutation assay with a tryptophan-requiring
strain of Escherichia coli WPrUvrA (Verspeek-Rip, 2002) . The test was
performed in two independent experiments in the presence and absence of
S9-mix (Aroclor-1254 induced rat liver S9-mix). The substance was tested
up to concentrations of 5 mg/plate in the absence and presence of
S9-mix. It precipitated on the plates at this dose-level. The bacterial
background lawn was not reduced at all concentrations tested and no
biologically relevant decrease in the number of revertants was observed.
The chromosome aberration study with melamine was performed according to
the US NTP standard protocol which is similar to the procedure outlined
in OECD testing guideline 473. Chinese hamstoer Ovary (CHO) cells were
used together with and without S9 mix prepared from the liver of
arochlor 1254 induced rats. Applied doses were 240, 270 and 300 µg per
plate. Mitomycin C and cyclophosphamide were used as positive controls.
Melamine was found to be non clastogenic in vitro. Considering the
higher molecular weight of melamine phosphate and that both are of
comparable solubility in water, this data is adequate to conclude that
melamine phosphate is not clastogenic in vitro.
Mutagenicity in mammalian cells in vitro was assessed in the mouse
lymphoma assay performed according the US National Toxicology Standard
Program which is comparable to OECD testing guideline 476 (McGregor
1988). Concentrations of
Considering the higher molecular weight of melamine phosphate and that
both are of comparable solubility in water, this data is adequate to
conclude that melamine phosphate is not mutagenic to mammalian cells in
Melamine was investigated in a micronucleus study in vivo
(Shelby 1993). Groups of five mice were administered the substance at 0,
500, 1000 or 2000 mg/kg
Dangerous Substance Directive (67/548/EEC)
The available studies are considered reliable and suitable for
classification purposes under 67/548/EEC. As a result the substance is
not considered to be classified for genotoxicity under Directive
67/548/EEC, as amended for the 28th time in Directive 2001/59/EC.
Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are reliable and suitable for
classification purposes under Regulation 1272/2008. As a result the
substance is not considered to be classified for genotoxicity under
Regulation (EC) No. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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