Registration Dossier

Ecotoxicological information

Toxicity to soil microorganisms

Currently viewing:

Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Poor description of the part of the publication on the inhibition of soil microorganisms.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Nitrification of Triazine Nitrogen
Author:
Hauck RD, Stephenson HF
Year:
1964
Bibliographic source:
Journal of agricultural and food chemistry, 12, 147-151

Materials and methods

Principles of method if other than guideline:
Perfusion of test substance solution through soil. Determination of produced NO3- from added ammonium, and possible inhibition by melamine (or cyanuric acid).
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Melamine (N-content: 66.5 %), reagent grade,
and also Cyanuric acid (N: 32.4 %) were tested.
Melamine was obtained from American Cyanamid Co.

Test organisms

Test organisms (inoculum):
soil

Study design

Total exposure duration:
165 d

Test conditions

Test temperature:
25 °C
Details on test conditions:
Silty clay loam was used, pH 8.2.
Nominal and measured concentrations:
Ca. 490 mg melamine / kg soil (10 mg N of melamine in 30 g soil).
And also: 10mg N of cyanuric acid in 30 g soil.
Perfusion was performed with and without 10 mg N of ammonium.

Results and discussion

Details on results:
Melamine (and cyanuric acid) perfusing with added ammonium slightly delayed the nitrification of ammonium by ca. 2 days.

Applicant's summary and conclusion

Conclusions:
Melamine only slightly inhibits nitrifying soil microorganisms.
Executive summary:

Melamine solutions (ca. 490 mg melamine/ kg soil) perfusing through silty clay loam soil slightly delayed the rate of nitrate formation from added ammonium by ca. 2 days.