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EC number: 255-449-7
CAS number: 41583-09-9
A liquid chromatographic method is described
for the determination of melamine in
beverages. Melamine is separated by column
chromatography using cation and anion
exchange resin and determined by ion-pair
liquid chromatography using an ODS column
and a mixture of acetonitrile and 0.05M
phosphate buffer (pH 3.0) containing 0.005M
sodium 1-laurylsulfate (1 + 4, v/v) as
mobile phase. Recoveries of melamine ranged
between 90.3 +/- 7.8 and 102.1 +/- 5.6% at
levels of 0.6 to 2.4 ppm in 4 kinds of
beverages. The quantitation limit was 2.5
micrograms melamine in 50 mL beverage. The
method was applied to the migration test of
melamine from melamine-formaldehyde resin
products to the beverages.
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