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Long-term toxicity to aquatic invertebrates

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Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly dissolved with 2 hours stirring. An aliquot of the test item (purity 73.3%) was weighed onto a sterile Teflon plate which was then placed directly into a sterile 2 L Schott bottle. Under laminar flow 2 L of sterile dilution water was added to the mixing vessels to reach a loading corresponding to the highest test concentration of 12 mg a.s./L.
The content of the vessel was stirred at 300 rpm for 2 h at room temperature (21 ± 1°C). The eluate was then used to begin a 50% serial dilution for the preparation of the remaining four test concentrations using sterilised dilution water.
The eluate was prepared 2 h under sterile conditions and replaced daily.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene. Specimens used in the test were bred in the laboratory of the Fraunhofer IME
- Age of parental daphnids: at least 3 weeks old
- Feeding during test: yes
- Food type: with concentrated suspensions of unicellular alga Desmodesmus subspicatus
- Amount: of 0.2 mg carbon/L per daphnid per day
- Frequency: at each media renewal (daily)

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: . Newborn Daphnia (between 4 and 23 hours old) were removed by wide-bore pipette (to avoid damage) and isolated in fresh dilution water for at least 1 hour prior to being added randomly to the test vessels containing the appropriate test or control media.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
1.0 mmol/L
Test temperature:
18.1 -20.9 °C
pH:
7.77 -9.91 mg/L
Dissolved oxygen:
6.8 - 11.0 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 0.51, 1.13, 2.48, 5.45, 12 mg/L
Mean measured test concentrations (TWA): -, 0.45, 1.03, 2.33, 5.27, 11.75 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass flasks, 100 ml, 90 ml
- Renewal rate of test solution: daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Cu-free tap water
- Alkalinity: 1.3-1.5 mmol/L
- Conductivity: 203.7 - 227.9 µS/cm
- Culture medium different from test medium:- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle of 16/8 hours
- Light intensity: 800 lx

EFFECT PARAMETERS MEASURED: The numbers of immobile daphnids were visually determined daily and the immobile daphnids were removed. Any abnormalities in appearance and behaviour were recorded. The newborn daphnids per beaker were counted and removed daily until day 10 (after day 10 newborn daphnids were counted and removed daily at each water renewal), abnormalities in condition (including male sex) or presence of winter eggs were checked and recorded. At study termination, length of the adults up to the highest treatment without significant mortality were measured by digital photography and image analysis and compared with the equally measured control animals.

Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
5.3 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
11.8 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 11.75 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks:
parental daphnids
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 11.75 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
growth
Remarks:
(body length)
Details on results:
- Mortality of parent animals: One parental daphnid died in the third highest treatment (2.33 mg/L; TWA) on day one and two animals died on day three in the second highest treatment (5.27 mg/L; TWA
- Type and number of morphological abnormalities: none
- Type and number of behavioural abnormalities: none
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Reason / purpose:
read-across source
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
5.3 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
11.8 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 11.75 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks:
parental daphnids
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 11.75 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
growth
Remarks:
(body length)

Description of key information

NOEC (21 d) = 5.3 mg a.i./L  (measured) related to reproduction.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
5.3 mg/L

Additional information

No chronic invertebrate studies on toxic effects of AES (C9 -11, 1 -2.5EO) Na (CAS 160901 -28 -0) are available. Therefore, this endpoint is covered by read across to a structurally related AES (C8 -10, 2.5EO) NH4 (CAS 68891 -29 -2).

Examining the toxic effects of AES (C8 -10, 2.5EO) NH4 a GLP study was performed according to OECD guideline 211 (BASF , 2013a). Under semi-static conditions a NOEC (21 d) of 5.3 mg a.i./L (measured) for Daphnia magna was observed. The NOEC value was based on reproduction.