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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Qualifier:
according to
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Qualifier:
according to
Guideline:
ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Details on preparation and application of test substrate:
- Method of mixing into soil: test item was weighed in directly on quartz sand, mixed and moistened. Then the test item was mixed into artifical soil.
- Controls: 4 replicates
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: laboratory rearing stock maintained in the testing facility
- Age at test initiation: at least two months old, but not older than 1 year with a clitellum (age of the worms did not idffer by more than four weeks)
- Weight at test initiation (range): between 350 and 450 mg

ACCLIMATION
- Acclimation period: 1day
- Acclimation conditions: same as test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
56 d
Test temperature:
20.1 - 23.9 °C
pH:
study inititation: 6.4 - 6.5
study end: 6.7 - 6.9
Moisture:
water content at study initiation: 24.2 - 25.4 %
water content at study end: 24.1 - 26.6 %
Details on test conditions:
TEST SYSTEM
- Test container (material, size): BELLAPLAST, 17 cm x 12.5 cm x 6 cm
- Amount of soil or substrate: 749 g moistened substrate ( corresponding to 600 g dry substrate)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate):
- 10 % sphagnum peat (close to pH 5.5-6.0; no visible plant remains, finely ground, air dry)
- 20 % kaolin clay (kaolinite content above 30%)
- 70 % industrial sand (fine sand dominant with more than 50% of the particles between 50 and 200 microns)
- Maximum water holding capacity (in % dry weight): 40.36%
- Pretreatment of soil: after mixing the components, i.e. before pre-moistening, 10 g air-dried finely ground cow manure per 1 kg substrate were added
- Storage (condition, duration): in a closed container

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Light intensity: 500 - 650 lux

EFFECT PARAMETERS MEASURED: Mortality, behavioural abnormalities and pathological symptoms were recorded after 28 days; number of offspring were determined after 56 days

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: yes
- Test concentrations: control, 150, 225, 338, 506 mg a.i./kg soil dry weight
- Results used to determine the conditions for the definitive study: no mortality after 28 days, mean weight change between 14.3 and 31.2%
Nominal and measured concentrations:
nominal test concentrations: control, 72, 129, 232, 417, 750 mg a.i./kg soil dry weight
Reference substance (positive control):
yes
Remarks:
Carbendazim
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
750 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: reproduction and body weight
Duration:
56 d
Dose descriptor:
EC50
Effect conc.:
> 750 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: none
- Total mass of adults at beginning of test:
- Changes in body weight of live adults (% of initial weight) at end of exposure period: 49.1, 44.4, 43.2, 52.3 and 58.5 % in the test item treatment groups at 72, 129, 232, 417 and 750 mg a.i./kg soil dry weight; in the control the average body weight change was 49.7 %
- Morphological abnormalities: none
- Behavioural abnormalities: none
Results with reference substance (positive control):
in a test conducted from 24 May to 20 Aug 2011 (report no. S11-01755) a statistically significant reduction in reproduction of 68% was observed.

Number of juvenile worms per vessel after 56 days of exposure to the test item

Texapon N 70
[mg a.i./kg sdw]

Number of juvenile worms
per replicate (n=10)

Mean value

±SD

CV
[%]

Difference to control
[%]

1

2

3

4

5

6

7

8

Control

107

108

110

118

168

124

113

147

124.4

21.9

17.6

 -

72

103

118

92

102

 -

 -

103.8

10.7

10.3

-16.6

129

85

128

104

71

 -

 -

97.0

24.7

25.5

-22.0

232

152

156

144

102

 -

 -

138.5

24.8

17.9

+11.3

417

102

105

124

138

 -

 -

117.3

16.9

14.4

-5.71

750

105

128

123

89

 -

 -

111.3

17.8

16.0

-10.5

SD = Standard Deviation                            sdw = soil dry weight         
CV = Coefficient of Variation

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Reason / purpose:
read-across source
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
750 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: reproduction and body weight
Duration:
56 d
Dose descriptor:
EC50
Effect conc.:
> 750 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality

Description of key information

NOEC (56 d) = 750 mg a.i./kg soil dw  for Eisenia fetida 

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
750 mg/kg soil dw

Additional information

Since no studies on toxicity to soil organisms are available for AES (C9-11, 1-2.5EO) Na (CAS 160901-28-0) a read-across approach from a comparable AES (C12 -14, 2EO) Na (CAS 68891 -38-3) was applied.

In this test the earthworm Eisenia fetida was exposed to different test concentrations of the AES (C12 -14, 2EO) Na mixture (CAS 68891-38-3). The study was performed according to OECD guideline 222 and GLP. The NOEC (56 d) for body weight change and reproduction was determined as 750 mg a.i./kg soil dry weight. Up to the highest test concentration (750 mg a.i./kg soil dry weight) no mortality was observed (BASF, 2012b).