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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Justification for type of information:
Please refer to the Category Approach Justification provided in Section 13
Qualifier:
according to
Guideline:
other: DIN 38412, part II
GLP compliance:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: LVA, Düsseldorf, Germany
- Age at study initiation (mean and range, SD): <= 24 h
- Feeding during test: none

Culture conditions
- Culture temperature: 20 ± 1 °C
- Photoperiod: 16 h diffuse light daily
- Type and amount of food: cells of the green algae Scenedesmus spec.
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
pH:
7.5-7.9
Nominal and measured concentrations:
Nominal test concentrations: control, 8, 16, 32, 64, 128, 256, 512, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

EFFECT PARAMETERS MEASURED: immobilisation after 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2
- Range finding study: yes
- Test concentrations: 0.1, 1, 10, 100, 1000 mg/L
Reference substance (positive control):
yes
Remarks:
Potassiumdichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
64 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
91 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
128 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
64 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
170 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
128 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
- Mortality of control: none
Results with reference substance (positive control):
EC50 (24 h) = 1.2 mg/L

Description of key information

EC50 (48 h) for Daphnia magna = 91 mg a.i./L (nominal, DIN 38412, part II) 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
91 mg/L

Additional information

No acute invertebrate studies on toxic effects of AES (C9 -11, 1 -2.5EO) Na (CAS 160901 -28 -0) are available. Therefore, a read-across approach from the structural comparable AES (C8 -10, 2.5EO) Na was applied.

This study was performed according to DIN 38412, part II for AES (C8 -10, 1 -2.5EO) Na. Under static conditions an EC50 (48 h) value of 91 mg a.i./L (nominal) for Daphnia magna was determined (Z&S, 1992).