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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The test substance, Centralit, was tested in acute immobilisation test on Daphnia magna.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
Since the test substance is poorly soluble in dilution water at concentrations needed for the test, the saturated solutions of the test substance in dilution water were prepared.
The full test was performed in range of test substance nominal concentrations 10 - 49 mg.L-1. Samples for analytical determination were taken at the beginning and at the end of the test.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
14.3 mg/L

Additional information

The analytical results showed that the test substance Centralit was stable in dilution water at conditions of the test, therefore the nominal concentrations were used for all evaluations and results.

The value EC50 (48h) for daphnia magna was used for C&L and for Chemical Safety Assessment.