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EC number: 275-702-5
CAS number: 71617-10-2
Table 3: Determined ECx-values for the test
Based on biomass (EbC)
Based on growthrate (ErC)*
* no data given for the confidence
Table 4: Study evaluation after 72
hours of exposure
Nominal concentration test substance [mg/L]
[integral of biomass]
% inhibition [-] or % increase [+] of growth compared to the control
Growth rate [1/d]
% inhibition [-] or increase [+] of growth rate compared to the control
Exponentially-growing cultures of a species of green algae were
exposed to six different concentrations of the test substance over
several generations under defined conditions. The test solutions were
incubated for a period of 72 h, during which the cell density in each
vessel was measured every 24 h. The inhibition of growth and growth rate
in relation to a control was determined after 72 h after exposure to the
test substance. The concentrations of the test substance which cause a
50 % reduction in growth (EbC50) and growth rate (ErC50)
relative to control were determined with 0.10 mg/L and 0.20 mg/L
respectively. The lowest concentration which causes reduction of the
growth in the tested algae species compared to the control within a 72-h
exposure period (=LOEC) was determined as 0.10 mg/L, whereas the highest
concentration not causing growth inhibition (=NOEC) was determined as
The 72h ErC50 value is 0.2 mg/L. The 72h NOEC value was determined to be 0.06 mg/L.
key study was performed with the freshwater green algal species Scenedesmus
subspicatus according to EU Method C.3 (1992) in a 72 -hour static
test. Six nominal concentrations of 0.031, 0.063, 0.13, 0.25, 0.5 and 1
mg/L were tested. Test concentrations were measured at the beginning and
end of the test. Results were based on the arithmetic mean of analytical
values. Measurements of replicates without algae showed that the
substance loss can be mainly attributed to adsorption to algae.
test substance concentration which caused a 50% reduction in biomass
(EbC50) and growth rate (ErC50) relative to control were determined at
0.10 mg/L and 0.20 mg/L, respectively. The lowest concentration which
causes reduction of the growth in the tested algae species compared to
the control within the 72-h-exposure period (=LOEC) was determined at
0.10 mg/L, whereas the concentration not causing growth inhibition
(=NOEC) was determined to be 0.06 mg/L.
second study probably conducted according to OECD
201 but as only a results page is available and thus no details on the
study are available the reliability of the study could not be assessed,
and therefore a reliability level of 4 (not assignable) was given to the
study. Nevertheless the study supports the findings in the key study.
The 72h-NOEC was determined to be 0.13 mg/L and the EC50(72h) was
determined to be 0.34 and 0.5 mg/L, respectively.
effects of the test substance on the growth of marine algae were not
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