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EC number: 275-702-5
CAS number: 71617-10-2
All relevant information on results is provided in sections "Test
results" and "Additional information on results".
The potential genotoxicity of isopentyl p-methoxycinnamate
was investigated using the HPRT assay in V79 cells of the Chinese
hamster, an in vitro assay system for the induction of mutations in
mammalian cells. The assay was performed according to OECD guideline no.
476 and EU Method B.17 and consisted of two independent experiments. In
the first experiment, the cells were exposed to the test item for 4 h
with and without metabolic activation. In the second experiment, cells
were treated with the test item for 24 h in the absence of metabolic
activation and for 4 h in the presence of metabolic activation.
In the first experiment, cells were exposed to the test item at
concentrations ranging from 18.8 to 2,500 µg/mL, and from 0.6 to 40
µg/mL, for tests with and without metabolic activation, respectively. In
the second experiment, the test without metabolic activation was
conducted with concentrations of the test item ranging from 1.3 to 80
µg/mL, while in the test with metabolic activation, a concentration
range of 18.8 to 2,500 µg/mL was used.
Accordingly, the highest concentration used in this study (2,500
µg/mL) corresponded to the highest test concentration of 10 µM that is
recommended in OECD guideline no. 476.
The dose range used in the two experiments was limited by
precipitation of the test item (in the first experiment, at 40 µg/mL
without metabolic activation, and in both experiments at 300 µg/mL with
metabolic activation) and cytotoxicity.
No substantial and reproducible, dose-dependent increase of the
mutation frequency was observed in both experiments with isopentyl
7,12-Dimethylbenzanthracene (DMBA) and ethylmethanesulphonate
(EMS) were used as positive controls for experiments with and without
metabolic activation, respectively. The positive control compounds
induced a distinct increase in mutant colonies and thus, demonstrated
the sensitivity of the test and the activity of the metabolic activation
In conclusion, it can be stated that under the experimental
conditions reported the test item did not induce gene mutations at the
HPRT locus in V79 cells.
Therefore, isopentyl p-methoxycinnamate is considered
to be non-mutagenic in this HPRT assay.
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