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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Study period:
no data
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Reference:
Composition 1
Guideline:
other: no data
GLP compliance:
no
Test material information:
Composition 1
Analytical monitoring:
not specified
Details on test solutions:
no details
Test organisms (species):
Daphnia sp.
Details on test organisms:
no details
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
no data
Dose descriptor:
EC0
Effect conc.:
1 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Dose descriptor:
EC100
Effect conc.:
31.6 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Dose descriptor:
EC50
Effect conc.:
5.6 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: EC50 = EC0/EC100
Validity criteria fulfilled:
not specified
Conclusions:
In the acute test with daphnia an EC0 of 1 mg/L and and EC100 of 31.6 mg/L was determined. The EC50 was determined by caclulating the geometric mean of the EC0 and EC100. The EC50 is 5.6 mg/L.
No details about the test design (duration, test system, etc.) are known.
Executive summary:

In the acute test with daphnia an EC0 of 1 mg/L and and EC100 of 31.6 mg/L was determined. The EC50 was determined by caclulating the geometric mean of the EC0 and EC100. The EC50 is 5.6 mg/L. No details about the test design (duration, test system, etc.) are known.

Description of key information

The test substance is acutely toxic to aquatic invertebrates (EC50 = 0.28 mg/L).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.28 mg/L

Additional information

The acute toxicity of the substance was studied in a valid GLP test according to EU Method C.2 (key study). The test was performed under static conditions over a period of 48 hours. The tested daphnia (5 animals per concentration, 2 replicates) were exposed to the test substance at 5 different concentrations (0.056, 0.11, 0.23, 0.45, and 0.9 mg/L) for 48 h.

The highest concentration which did not cause immobilisation after 48 h was 0.1 mg/L (= EC0, arithmetic mean), whereas the concentration that caused 100% immobilisation after 48 h (= EC100, arithmetic mean) was 0.76 mg/L. The geometric mean between the EC0 and the EC100 value, which is considered to represent the EC50 value, was determined at 0.28 mg/L. In the control, no immobilisation occurred at all test concentrations after both exposure periods, ensuring the validity of the test system. The second required validity criterion for the test, a dissolved oxygen concentration > 3 mg/L at the end of the test in control and test vessels, was also fulfilled.

The second acute study with daphnia was probably conducted similar to OECD 202 but as only a result page is available and thus no details on the study are available the reliability of the study could not be assessed and therefore a reliability level of 4 (not assignable) is given to this study. The EC50 (geometric mean of EC0 and EC100) was determined to be 5.6 mg/L.

The effects of the test substance on marine invertebrates were not studied.