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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 1971
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1971
Report Date:
1971

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
According to ref.: J.H. Kay et col., J.Soc.Cos.Chem. 13/281, 1962
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals / tissue source

Species:
rabbit
Strain:
other: albino
Details on test animals or tissues and environmental conditions:
No data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent no treatment
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted
Duration of treatment / exposure:
Group I - 168 h (not rinsed)
Group II - 2 sec (rinsed)
Group III - 4 sec (rinsed)
Observation period (in vivo):
168 h (at 1, 24, 48, 72, 96 and 168 h)
Number of animals or in vitro replicates:
Group I - 3
Group II - 3
Group III - 3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Group I: not rinsed; Group II: rinsed after 2 sec; Group III: rinsed after 4 sec
- Time after start of exposure: 2 sec and 4 sec

SCORING SYSTEM: according to ref.: J.H. Kay et col., J.Soc.Cos.Chem. 13/281, 1962

Results and discussion

Results of in vivo study
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 1 h
Score:
3.3
Reversibility:
fully reversible within: 24 h
Remarks on result:
other: overall score from all groups
Irritant / corrosive response data:
A slight irritation of the conjunctivae was observed during the few hours after application of pure 2-Ethylhexyl 4-methoxycinnamate. This
irritation is manifested by the presence of some more capillaries injected into the eye treated than on the eye not treated. No effect could
be detected 24 hours after application of the test substance. No effect of rinsing was observed.
Other effects:
No data

Any other information on results incl. tables

Observation time

Animal no.

Group I conjunctivae

Group II conjunctivae

Group III conjunctivae

Results

1 h

1

A2

A1

A2

3.3

1 h

2

A1

A2

A1

 

1 h

3

A2

A2

A2

 

24 h

1

 

 

 

0

24 h

2

 

 

 

 

24 h

3

 

 

 

 

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
No effects on eye irritation could be detected 24 h after application of 2-ethylhexyl 4-methoxycinnamate.
Executive summary:

The eye irritation potential of 2-ethylhexyl 4-methoxycinnamate, a close structural analogue of isopentyl p-methoxycinnamate, was evalauted in a Draize test in albino rabbits.

2-ethylhexyl 4-methoxycinnamate

was injected into the left eye of 3 albino rabbits without rinsing, the left eye of another 3 albino rabbits were rinsed after 2 and 4 seconds, resp. Cornea, iris, and conjunctivae were examined. A slight irritation of the conjunctivae was observed during the few hours after application of pure

2-ethylhexyl 4-methoxycinnamate

. This irritation is manifested by the presence of some more capillaries injected into the eye treated than on the eye not treated. No effect, however small, could be detected 24 h after application of the test substance.

In view of the close chemical relationship between isopentyl p-methoxycinnamate and Neo Heliopan AV, the negative findings obtained with

2-ethylhexyl 4-methoxycinnamate

provide further evidence for the non-eye-irritancy of isopentyl p-methoxycinnamate in rabbits.