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EC number: 254-052-6
CAS number: 38640-62-9
test substance did not induce mutant colonies above background in any
strain at any
The first main test (with S-9 mix,
maximum concentration 75 µg/mL) was dismissed and not continued for
chromosomal analysis. The reason was a reduction in the mitotic index at
75 µg/mL, higher than expected from pre-testing. Therefore, a second
main trial was initiated with a somewhat reduced upper concentration (60
µg/mL). Cytotoxic screening was relinquished for the second main test
because cytotoxicity had been demonstrated in prior screening.
No significant increase in chromosomal
damage was seen in cultures treated with diisopropylnaphthalene at any
dose level in either the presence or absence of metabolic activation.
Positive controls caused large,
statistically highly significant increases in chromosomal damage, thus
demonstrating the sensitivity of the test system and the efficiency of
the S-9 mix.
In this in-vitro cytogenetic test
diisopropylnaphthalene did not show any evidence of clastogenic activity.
Mean survival (day 0) in non-selective
medium of the solvent control in the absence of S-9 mix was 74 and 68 %
(Tab. 1 + 4), and in the presence of S-9 mix 72 - 88 % (Tab. 7 + 10).
In test 1 without S-9 mix (3-h
treatment), a statistically significant increase in mutant frequency
[MF] outside the historical control range was observed at the highest
dose (40 µg/ml). Mean day-0 rel. survival was 12% and day-2 cloning
efficiency CE was 19 % (Tab. 1 - 3). This effect can be explained by the
corresponding decrease in day-2 CE in non-selective medium (MF = CE
selective/CE non-selective). No substantive increase was seen in Test 2
under equivalent conditions, except 24-h exposure.
In test 1 with S-9 mix (3-h
treatment), a statistically significant increase in MF outside the
historical control range was observed at 20 µg/ml, but not at 50 µg/ml
(survival 14 %) (Tab. 7 and 9). But this effect was not reproduced in
the 2nd test.
Colony sizing demonstrated no
difference in the ratios small-to-large colonies from the solvent
control, while significant higher yields of small colonies were found in
the pos. controls.
was no reproducible increase in mutant frequency and no evidence of a
dose relationship over at least two dose levels. Based on the results of
this test, it can be concluded that DIPN did not demonstrate mutagenic
potential in this in-vitro gene mutation assay.
Diisopropylnaphthalene did not produce
any statistically significant increase in micronucleated polychromatic
decline of polychromatic erythrocytes associated with a concurrent
increase in normochromatic erythrocytes indicated some toxic effects of
diisopropylnaphthalene on erythropoiesis.
Genetic toxicity in vitro
Four valid tests are available to
assess the genotoxic potential of DIPN. Two bacterial reverse mutation
assays, one mammalian cell gene mutation assay and one in-vitro
mammalian chromosome aberration test were performed according or similar
to the respective OECD TG.
Leimbeck 1986 (Ames
In a bacterial reverse mutation assay
using four strains of S. typhimurium, there was no evidence of an
increase in revertant colonies above background in any strain and at any
concentration tested with and without metabolic activation. DIPN proved
to be negative in the Ames test under the test conditions used.
Kureha 1982 (bacterial
reverse mutation assay)
In a bacterial reverse mutation assay
using five strains of S. typhimurium and one strain of E. coli, there
was no evidence of an increase in revertant colonies above background in
any strain and at any concentration tested with and without metabolic
activation. Diisopropylnaphthalene proved to be negative in this
bacterial reverse mutation test under the test conditions used.
Huntington 1999 (mammalian
cell gene mutation (TK) assay)
The mutagenic potential of DIPN was
tested in an in vitro mammalian cell gene mutation assay using mouse
lymphoma L5178Y cells. There was no evidence of increases in mutant
frequency according to the criteria of the test for all test substance
concentrations tested without and with metabolic activation. It can be
concluded that DIPN does not demonstrate mutagenic potential under the
test conditions of this assay.
Huntington 1988 (mammalian
chromosomal aberration test)
In an in vitro mammalian chromosome
aberration test, DIPN did not demonstrate any increase in chromosomal
damage at any dose level either without or with metabolic activation.
There was no evidence of any clastogenic activity under the conditions
of the test used.
Genetic toxicity in vivo
The genotoxic potential of DIPN in
vivo was investigated in one valid test.
IBR 1986 (micronucleus
Under the conditions of the
micronucleus test performed, DIPN administered at 2 mL/kg bw did not
lead to any significant increase in the frequency of micronucleated
polychromatic erythrocytes compared to control values at any of the
three time point examined.
Short description of key information:
For bis(isopropyl)naphthalene (DIPN), five different tests on
genetic toxicity (bacterial and mammalian cell mutation, chromosomal
aberration, micronucleus test) have been performed. In all tests, there
was no evidence of any genotoxic potential.
Endpoint Conclusion: No adverse effect observed (negative)
No classification required (see discussion
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