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EC number: 231-901-9
CAS number: 7778-39-4
- 2/10 animals died within 10 days;
surviving animals appeared normal at study termination
In a Buehler closed patch study conducted
similarly to OECD Guideline 406 and in compliance with GLP, a group of
10 male Dunkin-Hartley guinea pigs were topically induced with arsenic
acid (0.4 mL) at a concentration of 25% w/v (first two doses) or 10% w/v
in deionised water (last dose) via occlusive patch for 6 hours, followed
by a rest period of 2 weeks and then with a challenge patch of 1% w/v.
Ten naive animals were applied with a challenge patch in the same
manner. Four animals in the positive control group were applied with
three induction patches of 0.3% w/v 2,4-dinitrochlorobenzene (DNCB) in
80% ethanol/deionised water followed by a challenge patch of 0.1% w/v
DNCB in acetone. The test concentrations for the main study were
determined from the range-finding studies conducted at concentrations of
25, 50, 75 and 100% w/v (study 1) and 0.1, 1, 10 and 25% w/v in
deionised water (study 2).
Topical induction indicated evidence of mild
to strong dermal reactions. No skin reactions were noted at the
challenge sites of the test or untreated control group animals at the 24
or 48-hour observations. Arsenic acid produced a 0% (0/20) sensitisation
rate and was considered to be a nonsensitiser to guinea pig skin.
Positive control exhibited evidence of sensitisation, thus confirming
the susceptibility of this group of animals to dermal sensitisation.
Under these test conditions, arsenic acid is
not classified according to the Annex VI of the Directive 67/548/EEC and
the CLP Regulation (EC) No. 1272/2008. However this study is not considered
as acceptable as it does not satisfy the guideline requirement for a skin
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