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EC number: 231-901-9
CAS number: 7778-39-4
For the determination of the effect concentration, the following
procedure is used:
A halflogarith graph is used, with on X the substance concentrations and
on Y the extinction values.
Calculation is made of the mean value of the extinction for test
concentrations at which the cultures where non affected (neither
stimulation nor inhibition) This is the point A . The allowed standard
deviation is 3%. The mean value of the extinction in the three test
cultures of the lowest toxic substance concentration is calculated. This
is the point B. The 3 points A, B, and A-3 % are reported on a
halflogarithm Y ax. The corresponding concentrations are a, b, and c.
"c" is the concentration that corresponds to the beginning of the cell
The toxicity of the substance di-sodiumhydrogenarsenate was tested on
the bacteria pseudomonas putida. The organisms were exposed to
different concentrations of the test substance, with dilution
factors from 20to214.The inhibitory effect of the
test substance on the organisms was measured through turbidimetric
measurement. Cell increase reduction is measured through primary
light absorption after correction with the dissolved substance
absorption and protection from diffuse reflexion. A graphic assessment
of the results was performed in order to derive the concentration at
which the test substance starts reduce the cell number increase, i.e.
when effects of more than 3% are observed.
The resulting EC3% is 18 mg/L for the tested substance.
This corresponds to 6.75 mg/L As element. (Calculation of the assessor).
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