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EC number: 231-901-9
CAS number: 7778-39-4
Table 1: Individual eye irritation scores
Time after dosing
Mean (24, 48 and 72 hours)
* Animals found dead
# Eyes washed 20 sec post-instillation
Ne: Not evaluated due to severity of
response; score considered to be maximum (2) for calculation of mean
NC: Not evaluated due to necrosis in
conjunctiva, score considered to be maximum (3) for calculation of mean
In an eye irritation study conducted
similarly to the OECD Guideline 405 and in compliance with GLP, 9 (8
males, 1 female) rabbits of the New Zealand White strain were exposed to
0.1 mL of undiluted Desiccant L-10 (arsenic acid) in their right eye
while the other eye corresponded to the control. Treated and control
eyes of six animals remained unwashed. Twenty seconds after
instillation, both eyes of the remaining three animals were rinsed for
one minute with lukewarm tap water. The eyes were observed at 1, 24, 48
and 72 hours and 7, 10, 14, 17 and 21 days after instillation and graded
according to the method of Draize (1959).
All nine animals exhibited moderate to
severe conjunctival irritation (redness, chemosis, discharge),
conjunctival necrosis, iridial damage and extensive corneal opacity
and/or ulceration. Effects were slightly less severe in the washed eyes.
1/3 animal with washed eyes was free of ocular irritation within 14 days
post-instillation. Remaining animals continued to exhibit severe
irritation (including corneal opacities) at termination of the study
(Day 21). All animals with non-washed eyes had severe irritation prior
to being found dead or prior to sacrifice for humane reasons on Day 9.
Mean individual scores at 24, 48 and 72 h after exposure for animals
with unwashed eyes were 3, 3, 4, 4, 3 for cornea score; 1.3, 1, 1.7, 2,
1 for iris score; 1, 1.3, 2.3, 1.3, 1.7 for conjunctivae score and 2, 2,
2.3, 3, 2 for chemosis score. Mean individual scores at 24, 48 and 72 h
after exposure for animals with washed eyes were 3, 1.3, 1 for cornea
score; 1.3, 1, 1 for iris score; 2, 2.7, 2.7 for conjunctivae score and
3, 2.3, 2.7 for chemosis score.
Under the test conditions, arsenic acid is
classified as ‘R41 Risk of serious damage to eyes’ according to the
criteria of Annex VI of the Directive 67/548/EEC and ‘Irreversible
effects on the eye (Category 1)’ in CLP Regulation (EC) No. 1272/2008.
This study is acceptable and satisfies the
guideline requirement for an eye irritation study (OECD 405).
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