Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented with minor deviations: no measured data was realized during the experiment.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Differential effects of arsenite and arsenate to Drosophila melanogaster in a combined adult/developmental toxicity assay
Author:
Goldstein S.H and Babich H.
Year:
1989
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Drosophila melanogaster were maintained into vials containing unamended and amended medium with arsenite and arsenate. Survival flies were counted at the end of the exposure period (3 weeks).
GLP compliance:
no
Application method:
contact

Test material

Constituent 1
Reference substance name:
Arsenate
IUPAC Name:
Arsenate
Constituent 2
Reference substance name:
Arsenite
IUPAC Name:
Arsenite
Details on test material:
- Name of test material (as cited in study report): Arsenite and Arsenate
- Molecular formula (if other than submission substance): NaAsO2 and Na2HAsO4
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No details on properties of test surrogate or analogue material

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
No details on sampling

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
No details on preparation and application of test substrate

Test organisms

Test organisms (species):
Drosophila melanogaster
Animal group:
Diptera (fruit fly (small))
Details on test organisms:
TEST ORGANISM
- Common name: Drosophila (wild type Oregon -R)
- Source: no data

ACCLIMATION
- Acclimation period: no data

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
3 wk
Remarks:
The cultures were maintained for 7 days and the survival flies wree scored. Viable adult flies were then discarded and the vials were maintained for another 2 weeks.
Post exposure observation period:
No post exposure observation period

Test conditions

Test temperature:
20°C
Humidity:
no data
Photoperiod and lighting:
no data
Details on test conditions:
TEST SYSTEM
- Test container (material, size): plastic vials (50 cm3)
- Amount of soil or substrate: no data
- No. of organisms per container (treatment): ten males and ten females
- No. of replicates per treatment group: 2
- No. of replicates per control: 2


OTHER TEST CONDITIONS
- Photoperiod: no data
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : survival, emergence

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study: no range finding study
Nominal and measured concentrations:
0, 0.1, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5 and 2.0 mM (nominal concentrations)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 wk
Dose descriptor:
EC50
Effect conc.:
ca. 0.21 other: mM
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
development
Remarks on result:
other: additionnal results for arsenite exposure
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
ca. 0.54 other: mM
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: additionnal results for arsenite exposure
Duration:
3 wk
Dose descriptor:
EC50
Effect conc.:
ca. 0.45 other: mM
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
development
Remarks on result:
other: results based on arsenate concentrations
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
ca. 0.79 other: mM
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: results based on arsenate concentrations
Details on results:
After 4 days of exposure a reduction in survival of about 10% was noted for flies exposed to 0.25 to 0.75 mM arsenite or arsenate and of about 15 and 25% for flies exposed to 1.0mM arsenate and arsenite, respectively.
Reduction in the yield of adult flies in the developmental exposure was, apparently, not merely a reflection of the acute toxicities of the arsenics to the parental flies during their 7 day exposure. Thus, 0.25 mM arsenite or arsenate was not significantly toxic to the parental flies but in the developmental exposure assay these concentrations reduced the yield of the flies by about 60 and 40% respectively.

See the following figures in section 'Illustration (picture/graph)':
Figure 1: Survival of Drosophila melanogaster after 7 days of exposure to medium amended with varied concentrations of arsenite or arsenate
Figure 2: Reduction in the yield of Drosophila melanogaster by various concentrations of arsenite and arsenate
Results with reference substance (positive control):
No results with reference substance
Reported statistics and error estimates:
The data points on the toxicity curves are presented as the arithmetic mean ± standard error of the mean (Figure 1 and 2)

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
Under the test conditions, the 7d-LC50 and the 3wk-EC50 were 0.54 mM and 0.21 mM respectively.
Executive summary:

In a 7d-acute contact toxicity study and a 3wk developmental toxicity test, Drosophila melanogaster were exposed to arsenite and arsenate administered on instant drosophila medium at an application rate of 0 (control), 0.1, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5 and 2.0 mM. The 7d-LC50 and 3wk-EC50 values were 0.54 mM and 0.21 mM rspectively, based on arsenite concentrations.

 

Results Synopsis:

 

Test organisms and test organism age: Drosophila melanogaster

Test type: contact

 

For acute contact:

 

7d-LC50:           0.54 mM        

3wk-EC50:        0.21 mM

Endpoint(s) Effected: survival, emergence