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EC number: 202-992-2
CAS number: 101-96-2
44PD, read-across is done from 7PPD. The chronic toxicity of 7PPD to
terrestrial plants was tested according to OECD Guideline 208
(Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test). Five
to seven different test item concentrations per species were tested
against a solvent control (acetone) with each of the six plant species.
of two monocot species, Allium cepa (onion) and Avena sativa (oat), and
four dicot species, Brassica napus (rape), Cucumis sativus (cucumber),
Lycopersicon esculentum (tomato) and Pisum sativum (pea) were planted in
a natural sandy loam soil (standard soil LUFA Sp2.3) immediately after
test item application and left to grow under controlled conditions for
up to 15 days. Each species was tested at 5 out of 7 test item
concentrations (dose response test) ranging from 7.8 to 500 mg 7PPD/kg
soil dry weight. Test item was dissolved in acetone and mixed into
portions of fine quarts sand. After complete evaporation of the solvent
the spiked sand was mixed into the soil. A control soil and a solvent
control soil were prepared by adding either pure sand or acetone spiked
sand (without test item) to soil aliquots. Plants were allowed to emerge
and grow for up to 15 days following 50% emergence of the control
plants. Soils were supplied with water or nutrient solution by bottom
watering. The test was performed in growth chambers equipped with
artificial lighting (16 hours per day) and air conditioning (air
temperature ranging from 22 to 27°C The pots were placed randomly at the
beginning of the test and were re-arranged weekly. At half-time of the
test seedlings were evaluated visually. Final counting, visual
evaluation, and harvest to determine shoot length and shoot fresh weight
took place at test end on day 14 or 15.
test item significantly reduced seedling emergence of A. cepa by 42% at
250 mg 7PPD/kg soil dw (195.1 mg 44PD/kg soil dw). At this concentration
none of the seedlings survived. Seedling emergence of P. sativum was
significantly reduced by 62% at 500 mg 7PPD/kg soil dw (390.1 mg 44PD/kg
soil dw). For all other tested species adverse effects were observed
neither on emergence nor on survival
evaluation revealed discoloured leaves at the highest test
length and shoot fresh weight of B. napus seedlings was significantly
reduced at concentrations of 15.6 and 62.5 mg 7PPD/kg soil dw,
respectively. After correction for molecular weight, his corresponds to
concentrations of 12.17 and 48.8 mg 44PD/kg soil dw, respectively.
Hence, B. napus was the most sensitive species with regard to shoot
length and weight.
For 44PD, read-across from 7PPD was done. For 7PPD, the chronic toxicity to terrestrial plants was tested according to OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test). Five to seven different test item concentrations per species were tested against a solvent control (acetone) with each of the six plant species. No significant effect on seedling emergence or mortality was observed for any of the six test species. Effects were observed for shoot length and fresh weight. Shoot length and shoot fresh weight of B. napus seedlings were significantly reduced at concentrations of 15.6 and 62.5 mg 7PPD/kg soil dw, respectively. After correction for molecular weight, this corresponds to 12.2 and 48.8 mg 44PD/kg soil dw. Hence, B. napus was the most sensitive species with regard to shoot length and weight.
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