Registration Dossier

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Read-across from 7PPD, see read-across justification for more information.
Reason / purpose:
read-across source
Species:
Allium cepa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Allium cepa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Allium cepa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
48.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot lenght
Remarks on result:
other: corrected for MW
Species:
Allium cepa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
48.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Species:
Avena sativa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 390.1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Avena sativa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 390.1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Avena sativa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot lenght
Remarks on result:
other: corrected for MW
Species:
Avena sativa
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Species:
Brassica napus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Brassica napus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Brassica napus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
6.1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot length
Remarks on result:
other: corrected for MW
Species:
Brassica napus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
24.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Species:
Cucumis sativus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Cucumis sativus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Cucumis sativus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
48.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot length
Remarks on result:
other: corrected for MW
Species:
Cucumis sativus
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
48.8 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Species:
Lycopersicon esculentum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Lycopersicon esculentum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Lycopersicon esculentum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
19.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot length
Remarks on result:
other: corrected for MW
Species:
Lycopersicon esculentum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
43.6 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Species:
Pisum sativum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
195.1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: corrected for MW
Species:
Pisum sativum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
>= 390.1 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: survival
Remarks on result:
other: corrected for MW
Species:
Pisum sativum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot length
Remarks on result:
other: corrected for MW
Species:
Pisum sativum
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
97.5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Remarks on result:
other: corrected for MW
Validity criteria fulfilled:
yes
Remarks:
The validity criteria were fulfilled as seedling emergence in the control was ≥ 70% and seedling survival in the control was ≥ 90% for all six test species. No phytotoxicity was observed in the controls.
Conclusions:
The chronic toxicity of 7PPD to terrestrial plants was tested according to OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test). Five to seven different test item concentrations per species were tested against a solvent control (acetone) with each of the six plant species. No significant effect on seedling emergence or mortality was observed for any of the six test species. Effects were observed for shoot length and fresh weight. Shoot length and shoot fresh weight of B. napus seedlings was significantly reduced at concentrations of 15.6 and 62.5 mg 7PPD/kg soil dw, respectively. Hence, B. napus was the most sensitive species with regard to shoot length and weight.
Executive summary:

For 44PD, read-across is done from 7PPD. The chronic toxicity of 7PPD to terrestrial plants was tested according to OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test). Five to seven different test item concentrations per species were tested against a solvent control (acetone) with each of the six plant species.

Seeds of two monocot species, Allium cepa (onion) and Avena sativa (oat), and four dicot species, Brassica napus (rape), Cucumis sativus (cucumber), Lycopersicon esculentum (tomato) and Pisum sativum (pea) were planted in a natural sandy loam soil (standard soil LUFA Sp2.3) immediately after test item application and left to grow under controlled conditions for up to 15 days. Each species was tested at 5 out of 7 test item concentrations (dose response test) ranging from 7.8 to 500 mg 7PPD/kg soil dry weight. Test item was dissolved in acetone and mixed into portions of fine quarts sand. After complete evaporation of the solvent the spiked sand was mixed into the soil. A control soil and a solvent control soil were prepared by adding either pure sand or acetone spiked sand (without test item) to soil aliquots. Plants were allowed to emerge and grow for up to 15 days following 50% emergence of the control plants. Soils were supplied with water or nutrient solution by bottom watering. The test was performed in growth chambers equipped with artificial lighting (16 hours per day) and air conditioning (air temperature ranging from 22 to 27°C The pots were placed randomly at the beginning of the test and were re-arranged weekly. At half-time of the test seedlings were evaluated visually. Final counting, visual evaluation, and harvest to determine shoot length and shoot fresh weight took place at test end on day 14 or 15.

The test item significantly reduced seedling emergence of A. cepa by 42% at 250 mg 7PPD/kg soil dw (195.1 mg 44PD/kg soil dw). At this concentration none of the seedlings survived. Seedling emergence of P. sativum was significantly reduced by 62% at 500 mg 7PPD/kg soil dw (390.1 mg 44PD/kg soil dw). For all other tested species adverse effects were observed neither on emergence nor on survival

Visual evaluation revealed discoloured leaves at the highest test concentrations.

Shoot length and shoot fresh weight of B. napus seedlings was significantly reduced at concentrations of 15.6 and 62.5 mg 7PPD/kg soil dw, respectively. After correction for molecular weight, his corresponds to concentrations of 12.17 and 48.8 mg 44PD/kg soil dw, respectively. Hence, B. napus was the most sensitive species with regard to shoot length and weight.

Description of key information

For 44PD, read-across from 7PPD was done. For 7PPD, the chronic toxicity to terrestrial plants was tested according to OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test). Five to seven different test item concentrations per species were tested against a solvent control (acetone) with each of the six plant species. No significant effect on seedling emergence or mortality was observed for any of the six test species. Effects were observed for shoot length and fresh weight. Shoot length and shoot fresh weight of B. napus seedlings were significantly reduced at concentrations of 15.6 and 62.5 mg 7PPD/kg soil dw, respectively. After correction for molecular weight, this corresponds to 12.2 and 48.8 mg 44PD/kg soil dw. Hence, B. napus was the most sensitive species with regard to shoot length and weight.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:
6.1 mg/kg soil dw

Additional information