Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 239-701-3
CAS number: 15625-89-5
Validity criteria followed:
This study was considered valid since the
following criteria were met:
• the frequency of cells with structural
chromosome aberrations in the vehicle controls was consistent with CIT
• The frequency of cells with structural
chromosome aberrations in the positive control tests was significantly
higher than that of the vehicle controls and consistent with CIT
An in vitro mammalian cell chromosomal
aberration study was carried out with trimethylolpropane triacrylate in
accordance with OECD TG 473, EU Method B.10, Japanese guidelines and EPA
OPPTS method 870.5375.
A preliminary experiment was carried out
with trimethylolpropane triacrylate 23.1, 45.7, 92.6, 185, 370, 740,
1480 and 2960 μg/mL both with and without S9 mix. In the main
experiment, primary cell cultured human peripheral blood from two donors
were incubated with Trimethylolpropane Triacrylate at the following
1) 0.78, 1.56, 3.13, 6.25, 12.5, 18.75, 25
and 37.5 μg/mL without S9 mix (first experiment);
2) 1.56, 3.13, 6.25, 12.5, 18.75, 25, 37.5
and 50 μg/mL with S9 mix (first experiment);
3) 3.13, 6.25, 9.38, 12.5, 18.75 and 28.13
μg/mL without S9 mix (second experiment);
4) 9.38, 18.75, 28.13, 37.5, 50 and 75 μg/mL
with S9 mix (second experiment).
Cytotoxicity was observed at all
concentrations of the preliminary experiment. Hence this study was
rejected. Cytotoxicity ranged from 45 to 100% in the first experiment
and 26 to 83% in the second experiment at concentrations ≥ 12.5 μg/mL.
Statistically significant and
concentration-related increases in the frequency of cells with
structural chromosomal aberrations were noted at concentrations ≥ 9.38
μg/mL with metabolic activation. Statistically significant increases in
the frequency of cells with structural chromosomal aberrations were
noted at concentrations at and above 28.13 μg/mL.
In conclusion trimethylolpropane triacrylate
induced chromosome aberrations in cultured human lymphocytes both with
and without metabolic activation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again