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EC number: 208-288-1 | CAS number: 520-26-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Hesperidin has shown in a test according to OECD 301B to be readily biodegradable. As degradation in the toxicity flask was 72% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 37.6 mg/L”.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Hesperidin has shown in a test according to OECD 301B to be readily biodegradable. Degradation behaviour of positive control and toxicity control was acceptable. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence. If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 72% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 37.6 mg/L”. Ready degradability is defined in the guidelines as degradation surpassing 60% within 10 days after reaching a level of 10%. Therefore, the test item Hesperidin can be considered as “readily biodegradable”, as 75 % degradation were reached at the end of the 10-day-window.. No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.
Furtheron an OECD 301B test was performed on Flavonoides 90% Micro (consisting of 90% diosmin and 10% hesperidin) which also showed ready biodegradability, reaching 73% degradation following the 1 -day window and 100% after 28 days. Diosmin is structurally very similar to hesperidin and is obtained by oxidation of the central flavonoide ring, thus having just 2 hydrogen atoms less than hesperidin. Hence, these results do confirm the similarity of diosmin and hesperidin, both being flavonoides sharing the same carbohydrate moiety and support the ready biodegradability of this group of chemicals.
In accordance with column 1 of REACH (REGULATION (EC) No 1907/2006) Annex IX, further studies on surface water simulation testing do not need to be conducted as the substance hesperidin is readily biodegradable (80% biodegradation within 28 days).
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