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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1992-06-23 to 1992-06-25
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Directive 67/548/EEC, 1992
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: Stirring using Ultra-Turrax: The test item was directly weighted into water (1 L) and was treated 60 seconds at 8000 rounds. In all weighted samples not dissolved residues of test item stuck (gummy-like) at the surface of the used glass equipment.
- Controls: M4 medium according to Elendt, version of BGA (1992)
- Dilution medium: M4 medium according to Elendt, version of BGA (1992)
Test organisms (species):
Daphnia magna
Details on test organisms:
- Organism: Daphnia magna Straus, parthenogenetic females
- Strain: Clones from Bundesgesundheitsamt Berlin
- Test animals: laboratory breed from Bayer AG Leverkusen
- Age: 6 - 24 hours
- Medium: M4 according to Elendt, version of BGA (1992)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
14.8 °dH (M4 medium)
Test temperature:
20.6 - 21.0 °C
pH:
7.8 - 8.5
Dissolved oxygen:
7.2 - 8.8
Nominal and measured concentrations:
Nominal concentrations: 62.5, 125, 250, 500, 1000 mg/L.
No measured values.
Details on test conditions:
TEST SYSTEM
- Test vessel: cylindric test vessel, diameter 4.0 cm, height: 6.5 cm
- Amount of test medium: 20 mL
- Aeration: no
- Application of test item: direct application
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- M4 medium according to Elendt, version of BGA (1992)
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
253 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Probit analysis, confidence interval: 209 - 324 mg/L
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
193 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Probit analysis, confidence interval: 145-313 mg/L
Details on results:
Test item concentration (mg/L) / immobilized daphnids after 24 and 48 h (absolut) and after 24 and 48 h (%), dissolved oxygen (mg/L) / pH (48 h) / Temperature, °C (48 h):
- control / 0, 0, 0, 0 / 7.6 / 7.8 / 20.6
- 62.5 mg/L / 0, 0, 0, 0 / 7.3 / 7.9 / 20.7
- 125 mg/L / 2, 7, 10, 35 / 7.4 / 7.9 / 20.7
- 250 mg/L / 7, 12, 35, 60 / 7.2 / 8.0 / 20.7
- 500 mg/L / 20, -, 100, - / 8.7* / 8.5* / 21.0*
- 1000 mg/L / 20, -, 100, - / 8.8* / 8.5* / 21.0*

* These values were determined after 24 h due to the early immobilization of the daphnids.

The determination of the test item's water solubility was not feasible. After mixing the test item with water there was an immediate reaction. The test item and/or its hydrolysis products precipitated as a solid on the surface of the glass flask. In the aqueous phase neither the test item nor its hydrolysis products could be detected by the analysis method SFC being typical for the test item's analysis. Based on the chemical structure of the test item, it was considered that the hydrolysis degradation products reacted to high-molecular products.

For the preparation of the test media, the media was removed from the test vessel during continous stirring of the magnetic stirrer.
Results with reference substance (positive control):
Reference substance concentration (mg/L) / immobilized daphnids after 24 and 48 h (absolut) and after 24 and 48 h (%), dissolved oxygen (mg/L) / pH (48 h) / Temperature, °C (48 h):
- 0.5 mg/L / 0, 0, 0, 0 / 8.1 / 8.0 / 20.7
- 0.9 mg/L / 0, 3, 0, 15 / 7.0 / 8.0 / 20.7
- 1.6 mg/L / 10, 20, 50, 100 / 7.2 / 8.1 / 20.7
- 2.8 mg/L / 12, 20, 60, 100 / 7.0 / 8.1 / 20.7
- 5.0 mg/L / 20, -, 100, - / 8.6* / 8.2* / 21.1*

* These values were determined after 24 h due to the early immobilization of the daphnids.
Reported statistics and error estimates:
- Analysis after 24 h: Correlation coefficient: 0.997
- Analysis after 48 h: Correlation coefficient: 0.959

EC values after 24 h and confidence intervals:

- EC05: 113 mg/L (72 - 144 mg/L)

- EC10: 135 mg/L (94 - 167 mg/L)

- EC50: 253 mg/L (209 - 324 mg/L)

- EC90: 474 mg/L ( 361 - 802mg/L)

- EC95: 566 mg/L (415 - 1060 mg/L)

EC values after 48 h and confidence intervals:

- EC05: 61 mg/L (12 - 94 mg/L)

- EC10: 78 mg/L (23 - 112 mg/L)

- EC50: 193 mg/L (145 - 313 mg/L)

- EC90: 474 mg/L ( 299 - 2630 mg/L)

- EC95: 612 mg/L (354 - 4990 mg/L)

Validity criteria fulfilled:
yes
Conclusions:
The EC50 (48 h) was calculated to be 193 mg/L with confidence limits of 145 and 313 mg/L. The EC100 (48 h) was calculated to be 500 mg/L.
Executive summary:

The acute toxicity of the test item to Daphnia magna was tested under static conditions for 48 hours in accordance with EU method C.2. The following nominal concentrations were tested: 62.5, 125, 250, 500, 1000 mg/L. The EC50 (48 h) was calculated to be 193 mg/L with confidence limits of 145 and 313 mg/L. The EC100 (48 h) was calculated to be 500 mg/L. The EC50 (24 h) was calculated to be 253 mg/L with confidence limits of 209 and 324 mg/L. The EC100 (24 h) was calculated to be 500 mg/L.

Description of key information

The EC50 (48 h) was calculated to be 193 mg/L with confidence limits of 145 and 313 mg/L for daphnia magna. The EC100 (48 h) was calculated to be 500 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
193 mg/L

Additional information

The acute toxicity of the test item to Daphnia magna was tested under static conditions for 48 hours in accordance with EU method C.2. The following nominal concentrations were tested: 62.5, 125, 250, 500, 1000 mg/L. The EC50 (48 h) was calculated to be 193 mg/L with confidence limits of 145 and 313 mg/L. The EC100 (48 h) was calculated to be 500 mg/L. The EC50 (24 h) was calculated to be 253 mg/L with confidence limits of 209 and 324 mg/L. The EC100 (24 h) was calculated to be 500 mg/L.

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