1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1988-19-05 to 1988-09-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

Study with detailed documentation; no OECD guideline but national guideline followed

Qualifier:

according to guideline

Guideline:

other: Letale Wirkung beim Zebrabaerbling - Brachydanio rerio - LC0 / LC50 / LC100; 48 - 96 Stunden, Umweltbundesamt Berlin, Stand: Mai 1984

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
Before the beginning of the test the test solution was treated (8000 rounds) with "Ultra-Turrax" for 60 minutes. The test item settled on the bottom of the flask or, respectively swam on the surface of the test medium. Further not dissolved parts were observed after trying to disperse with Ultra-Turrax.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name, strain: Brachydanio rerio Hamilton - Buchnan
- Source: Westaquarium / Bad Lauterberg / Germany
- Date of birth: 10.05.1988
- Length (measured at the end of the study): 2.5 - 3.5 cm

ACCLIMATION
- No medical pre-treatment
- No mortality observed.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

13.5 (Grad dh)

Test temperature:

- control: 20.6 °C - 22.5 °C,
- 50 mg/L solution: 20.2 °C - 22.1 °C
- 70.7 mg/L solution: 20.1 °C - 22.2 °C
- 100 mg/L solution: 20.2 °C - 22.3 °C
- 141 mg/L solution: 20.3 °C - 22.1 °C
- 200 mg/L solution: 20.2 °C - 22.1 °C
- 283 mg/L solution: 20.2 °C - 22.2 °C
- 400 mg/L solution: 20.3 °C - 22.2 °C
- 566 mg/L solution: 20.3 °C - 21.8 °C

pH:

- control: 6.7 - 7.0
- 50 mg/L solution: 6.8 - 7.0
- 70.7 mg/L solution: 6.9 - 7.2
- 100 mg/L solution: 6.8 - 7.1
- 141 mg/L solution: 6.8 - 7.2
- 200 mg/L solution: 5.6 - 7.1
- 283 mg/L solution: 6.0 - 7.3
- 400 mg/L solution: 4.8 - 7.3
- 566 mg/L solution: 7.2 - 7.3

Dissolved oxygen:

- control: 8.2 - 9.2 mg/L (oxygen saturation: 95.8 - 105.1 %)
- 50 mg/L solution: 8.5 - 9.4 mg/L (oxygen saturation: 99.1 - 106.7 %)
- 70.7 mg/L solution: 8.4 - 9.4 mg/L (oxygen saturation: 97.9 - 106.5 %)
- 100 mg/L solution: 7.7 - 9.2 mg/L (oxygen saturation: 90.6 - 104.4 %)
- 141 mg/L solution: 7.6 - 9.1 mg/L (oxygen saturation: 88.6 - 103.5 %)
- 200 mg/L solution: 8.3 - 9.3 mg/L (oxygen saturation: 97.4 - 105.6 %)
- 283 mg/L solution: 1.3 - 9.3 mg/L (oxygen saturation: 15.3 - 105.6 %)
- 400 mg/L solution: 5.0 - 9.2 mg/L (oxygen saturation: 58.9 - 104.7 %)
- 566 mg/L solution: 8.3 - 9.1 mg/L (oxygen saturation: 97.0 - 103.5 %)

Nominal and measured concentrations:

Main test: 50; 70.7; 100; 141; 200; 283; 400 and 566 mg/L (nominal).
No analytical measurements.

Details on test conditions:

TEST SYSTEM
- Aquaria: 300 x 135 x 200 mm
- Amount of test medium: 5 L
- Aeration: yes

TEST MEDIUM / WATER PARAMETERS
- Prepared dringling water from Leverkusen (inspection date: 05.08.1988)
- Ca/Mg ratio: 63.4 mg/L : 11.7 mg/L
- K/S 4.3 - mmol/L: 0.15
- direct addition of test item

TEST CONCENTRATIONS
- Results of the pre-test used to determine the conditions for the definitive study: LC0 = 70.7 mg/L; LC100 = 200 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

70.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

566 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

199.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: confidence interval: 158.77 - 249.93 mg/L

Details on results:

Mortality (cumulative, %) after 0, 24, 48, 72 , 96 h:
- control: 0 %, 0 %, 0 %, 0 %, 0 %
- 50 mg/L solution: 0 %, 0 %, 0 %, 0 %, 0 %
- 70.7 mg/L solution: 0 %, 0 %, 0 %, 0 %, 0 %
- 100 mg/L solution: 0 %, 0 %, 0 %, 0 %, 10 %
- 141 mg/L solution: 0 %, 40 %, 40 %, 40 %, 40 %
- 200 mg/L solution: 0 %, 30 %, 30 %, 30 %, 30 %
- 283 mg/L solution: 0 %, 80 %, 80 %, 80 %, 80 %
- 400 mg/L solution: 0 %, 90 %, 90 %, 90 %, 90 %
- 566 mg/L solution: 0 %, 100 %

Other effects after 2, 24, 48, 72 , 96 h:
- control: normal behaviour
- 50 mg/L solution: normal swimming behaviour
- 70.7 mg/L solution: normal swimming behaviour
- 100 mg/L solution: normal swimming behaviour
- 141 mg/L solution: normal swimming behaviour
- 200 mg/L solution: normal swimming behaviour
- 283 mg/L solution: 9 animals were inactively and lethargic; inactive and lethargic swimming behaviour; normal swimming behaviour; normal swimming behaviour; normal swimming behaviour
- 400 mg/L solution: 7 animals were inactively and lethargic, 1 animal was motionless, after touching uncontrolled swimming behaviour; normal swimming behaviour; inactive and lethargic swimming behaviour;
- 566 mg/L solution: 3 animals showed normal swimming behaviour, 1 animal had a lethargical swimming behaviour, 2 animals were motionless, after touching uncontrolled swimming behaviour

Remarks:
In the course of the test oxidation processes of the test item (aldehyde -> acid) were expected. These processes were considered to result in a temporary decrease of dissolved oxygen. Such observations were made during the test at concentrations 283 mg/L and 400 mg/L.
The results were calculated using Probit analysis.

Conclusions:

The LC50 was determined to be 199.2 mg/L.

Executive summary:

The study was performed in order to evaluate the toxic potential of the test item towards freshwater fish, using the species Danio rerio in accordance with a correspondent guideline of the Umweltbundesamt Berlin. Ten fish were exposed to the test item for 96 hours in a static test. The following concentrations were tested: 50; 70.7; 100; 141; 200; 283; 400 and 566 mg/L. After 24 h in the141 mg/L solution 40 % mortality was observed. In the control, none of the fish showed signs of morbidity at the end of the test. The pH and the oxygen values were recorded. Due to the test substance's properties a temporary decrase of dissolved oxygen in the test medium at 283 mg/L and 400 mg/L concentration was observed. The LC0, LC100 and LC50 were determined to be 70.7, 566.0 and 199.2 mg/L, respectively.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1991-03-18 to 1991-03-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

GLP study, no OECD guideline but national guideline followed

Qualifier:

according to guideline

Guideline:

other: "Letale Wirkung beim Zebrabaerbling - Brachydanio rerio- " (LC0, LC50, LC100; 48-96 Stunden), Verfahrensvorschlag: Umweltbundesamt Berlin, Stand Mai 1984, SOP 4.002

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Application: direct addition

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Name: Zebrabaerbling (Brachydanio rerio Hamilton Buchnan), SOP 4.002
- Source: West-Aquarium (Bad Lauterberg)
- Date of birth: 12.11.1990
- Delivery date: 29.01.1991
- Length: 2.5 - 3.5 cm
- Mortality: <2 %
- Medium: synthetic freshwater according to ISO guideline

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

13.2 °dH

Test temperature:

- control: 20.9 - 21.7 °C
- 31.06 mg/L solution: 21.0 - 21.7 °C
- 100 mg/L solution: 20.7 - 21.8 °C
- 316 mg/L solution: 21.0 - 21.6 °C
- 1000 mg/L solution: 21 - 21.3°C

pH:

- control: 7.5 - 7.9
- 31.06 mg/L solution: 7.6 - 7.9
- 100 mg/L solution: 7.6 - 7.9
- 316 mg/L solution: 7.9 - 8.1
- 1000 mg/L solution: 7.9 - 8.4

Dissolved oxygen:

- control: 8.3 - 8.8 mg/L (%-saturation: 96 - 101.1 %)
- 31.06 mg/L solution: 7.6 - 8.9 mg/L (%-saturation: 90.9 - 102.5 %)
- 100 mg/L solution: 8.1 - 8.8 mg/L (%-saturation: 93.8 - 100.8 %)
- 316 mg/L solution: 7.6 - 8.8 mg/L (%-saturation: 88 - 101.4 %)
- 1000 mg/L solution: 8.6 - 8.8 mg/L (%-saturation: 99.5 - 101.4 %)

Nominal and measured concentrations:

31.6; 100; 316; 1000 mg/L (nominal concentrations)
No measured concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium 300 x 135 x 200 mm
- Medium amount : 5 L
- Aeration: yes

TEST MEDIUM / WATER PARAMETERS
- Dilution water: freshwater according to ISO guideline

TEST CONCENTRATIONS
- Test concentrations: 31.6; 100; 316; 1000 mg/L (nominal concentrations)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality (cumulative, %) after 0, 24, 48, 74, 96 hours:
- control: 0 %, 0 %, 0 %, 0 %, 0 %
- 31.06 mg/L solution: 0 %, 0 %, 0 %, 0 %, 0 %
- 100 mg/L solution: 0 %, 0 %, 0 %, 0 %, 0 %
- 316 mg/L solution: 0 %, 0 %, 20 %, 30 %, 40 %
- 1000 mg/L solution: 0 %, 100 %

Other effects after 24, 48, 74, 96 hours:
- 100 mg/L solution: inactive and lethargic swimming behaviour; inactive and lethargic swimming behaviour; inactive swimming behaviour; inactive swimming behaviour
- 316 mg/L solution: inactive and lethargic swimming behaviour after 2 h; inactive and lethargic swimming behaviour of 4 animals; motionless, after touching uncontrolled swimming behaviour of 3 animals; anormal swimming behaviour of 3 animals; after 48 h: 8 animals were motionless and showed after touching uncontrolled swimming behaviour; after 72 h 7 animals were motionless and after touchig uncontrolled swimming behaviour was observed; after 96 h 2 animals were motionless and showed after touching uncontrolled swimming behaviour, 4 animals showed inactive and lethargic swimming behaviour
- 1000 mg/L solution: after 2 hours 3 aminls showed inactive and lethargic swimming behaviour, 4 animals were motionless and after touching uncontrolled swimming behaviour was observed, 3 animals showed anormal swimming behaviour

Weights of the 10 fish at the following concentrations:
- control: 2.27 g
- 31.6 mg/L solution: 2.47 g
- 100 mg/L solution: 2.3 g
- 316 mg/L solution: 2.54 g
- 1000 mg/L solution: 3.00 g

Other observations:
- In solutions of all test concentrations undissolved parts of the test item (oily drops on the test mediums's surface) remained. After 15 minutes these residues coloured white.
- In the test solution with the concentration 100 mg/L differences in the temperature range outlined in the guideline occured.

Conclusions:

The LC0 and LC100 were determined to be 100 and 1000 mg/L, respectively.

Executive summary:

The study was performed in order to evaluate the toxic potential of the test item towards freshwater fish, using the species Danio rerio in accordance with a corresponding guideline of the Umweltbundesamt Berlin. Ten fish were exposed to the test item for 96 hours in a static test. The following concentrations were tested: 31.6, 100, 316 and 1000 mg/L. After 48 h in the 316 mg/L solution 20 % mortality was observed. In the control, none of the fish showed signs of morbidity at the end of the test. The pH and the oxygen values were recorded. The LC0 and LC100 were determined to be 100 and 1000, respectively.

Description of key information

The LC50 was determined to be 199.2 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

199.2 mg/L

Additional information

Key study:

The study was performed in order to evaluate the toxic potential of the test item towards freshwater fish, using the species Danio rerio in accordance with a correspondent guideline of the Umweltbundesamt Berlin. Ten fish were exposed to the test item for 96 hours in a static test. The following concentrations were tested: 50; 70.7; 100; 141; 200; 283; 400 and 566 mg/L. After 24 h in the141 mg/L solution 40 % mortality was observed. In the control, none of the fish showed signs of morbidity at the end of the test. The pH and the oxygen values were recorded. Due to the test substance's properties a temporary decrase of dissolved oxygen in the test medium at 283 mg/L and 400 mg/L concentration was observed. The LC0, LC100 and LC50 were determined to be 70.7, 566.0 and 199.2 mg/L, respectively.

Supporting study:

The study was performed in order to evaluate the toxic potential of the test item towards freshwater fish, using the species Danio rerio in accordance with a corresponding guideline of the Umweltbundesamt Berlin. Ten fish were exposed to the test item for 96 hours in a static test. The following concentrations were tested: 31.6, 100, 316 and 1000 mg/L. After 48 h in the 316 mg/L solution 20 % mortality was observed. In the control, none of the fish showed signs of morbidity at the end of the test. The pH and the oxygen values were recorded. The LC0 and LC100 were determined to be 100 and 1000 mg/L, respectively.