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EC number: 212-782-2 | CAS number: 868-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-12-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Purity not specified but typically ca. 98.9 %
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The material of a necessary amount is dissolved to the dilution water, and examination field liquid 10,000 mg/L is adjusted.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- -Test Organisms:
a) Size (length and weight): length = 20 - 21 mm;
weight= 0.13 - 0.15 g
b) Supplier / Source: obtained from commercial hatcheries - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 55.6 mg/L
- Test temperature:
- 23.9 - 24.4 degree C
- pH:
- 7.7
- Dissolved oxygen:
- 5.5-8.2
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 100 mg/L- nominal
101 mg/L-TWM measured - Details on test conditions:
- -Test Conditions:
a) Dilution Water Source: Dechlorination water
b) Dilution Water Chemistry: hardness= 55.6 mg/L CaCO3, pH=7.7, chlorine concentration < 0.02 mg/L
c) Exposure Vessel Type: 2.5 L test solution in a 3L-glass vessel
d) Nominal Concentrations (as mg/L): 100
e) Vehicle / Solvent and Concentrations: Not used
f) Stock Solutions Preparations and Stability: The material of a necessary amount is dissolved to the dilution water, and examination field liquid 10,000 mg/L is adjusted.
g) Number of Replicates: 2
h) Fish per Replicates: 10
i) Renewal Rate of Test Water: water renewal; 48 hours
j) Water Temperature: 23.9 - 24.4 degree C
k) Lighting Condition: 16:8 hours; light-darkness cycle
l) Feeding: Not feeding-Method of Analytical Monitoring: The tested concentrations were measured at 0 hour and 48 hours (in advance of test solution exchange) by gaschromatography method. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- See Table 2.
- Reported statistics and error estimates:
- -Statistical Method:
a) Data Analysis: Not described
b) Method of Calculating Mean Measured Concentrations (i.e.arithmetic mean, geometric mean, etc.): Time-weighted means
- Measured Concentrations (as mg/L): See Table 1
- Water chemistry in test (O2, pH) in the control and one concentration where effects were observed: pH 6.8-7.5; DO =5.5-8.2 mg/L(Oxygen saturation level >=60 %)
- Cumulative Mortality: See Table 2
- Stastical Result: Not described - Sublethal observations / clinical signs:
Table 1: Measured concentrations (as mg/L):
Nominal conc. Measured conc. (mg/L) (Percentage of (mg/L)nominal)
-----------------------------------------------------------
0 hour 48 hours Time-weighted mean
-----------------------------------------------------------
Control n.d. n.d. n.d.
-----------------------------------------------------------
100 103(103) 99.1(99.1) 101(101)
-----------------------------------------------------------
n.d.: < 2.50 mg/L
Table 2: Cumulative Mortality:
Nominal conc. Cumulative number of dead fish
(mg/L) (Percent mortality)
------------------------------------------------------------
0 hour 24 hours 48 hours 96 hours
------------------------------------------------------------
Control 0(0) 0(0) 0(0) 0(0)
------------------------------------------------------------
100 0(0) 0(0) 0(0) 0(0)
------------------------------------------------------------- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 (96h) fish is >100 mg/L.
- Executive summary:
The acute aquatic toxicity to fish of 2 -hydroxyethyl methacrylate was tested in a 96-hour toxicity study in a limit test acc. OECD 203 with orange killifish (Oryzias latipes) The test was conducted under semi-static conditions (renewal at 48 hours) with 2 replicates of ten fish per replicate and at nominal concentration of 100 mg/L and a study control with water.
Aqueous samples were measured for analytical verification of test concentrations at 0 and 48 hours. The resulting time-weighted mean measured concentrations were non detectable (control) and 101 mg/L.
No fish mortality or sublethal effects were observed during the exposure.
In this test LC50 (96h) fish is >100 mg/L.
Reference
Description of key information
In the key study 2-hydroxyethyl methacrylate was tested in a limit test acc. OECD 203 under semi-static conditions with Oryzias latipes (renewal at 48 hours) with 2 replicates of ten fish per replicate and at nominal concentration of 100 mg/L and a study control with water.
Aqueous samples were measured for analytical verification of test concentrations at 0 and 48 hours. The resulting time-weighted mean measured concentrations were non detectable (control) and 101 mg/L.
No fish mortality or sublethal effects were observed during the exposure.
LC50 (96h) fish is >100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 100 mg/L
Additional information
Five acute freshwater fish studies are available for 2-hydroxyethyl methacrylate with three considered reliable for assessment of this endpoint. Two studies reliable were conducted with the orange killifish (Oryzias latipes), while the other study was conducted with the fathead minnow (Primephales promelas). These studies were found to be of good quality and assigned a Klimisch score of one or two. The two orange killifish studies were conducted following standard OECD guidelines and under GLP. One study was conducted under semi-static conditions for 96 hours and the second study conducted under flow-through conditions for 14-days. The fathead minnow study was conducted under flow-through conditions for 96 hours and followed ASTM guidelines but not under GLPs. The study was conducted under flow-through conditions for 96 hours. The studies concluded that the 96-hour and 14-day LC50 values for the ricefish were both >100 mg/L and the 96-hour LC50 for the fathead minnow was 227 mg/L. Since these toxicity results were from studies with different fish species and/or varying exposure periods, the lowest of the three values (>100 mg/L) was selected as the key parameter for this endpoint.
The remaining two fish studies (supporting studies) with the golden ide (Leuciscus idus melanotus) and the goldfish (Carassius auratus) were not considered reliable for assessment. The studies were conducted under static conditions and did not follow standard test guidelines. The golden ide study reported a 48-hour LC50 value of 360 mg/L and the goldfish study reported a 72-hour LC50 value of 374.5 mg/L, respectively.
No study is available for marine fish.
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