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EC number: 482-020-3
CAS number: -
TABLE 1. EXPERIMENT 1
MEAN NUMBER OF COLONIES/3 REPLICATE PLATES (+/- SD)
Without S-9 MIX
Solvent control: 0.1 ml ethanol
1 The S9-mix contained 5% (v/v) S9 fraction
a Bacterial background lawn absent
Unoxol™ 3,4 Dialdehyde was tested in the Salmonella typhimurium reverse
mutation assay with four histidine-requiring
strains of Salmonella typhimurium (TA1535, TA1537, TA100 and TA98) and
in the Escherichia coli reverse mutation assay
with a tryptophan-requiring strain of Escherichia coli (WP2uvrA). The
test was performed in two independent experiments in
the presence and absence of S9-mix (rat liver S9-mix induced by a
combination of phenobarbital and β-naphthoflavone).
The study procedures described in this report were based on the most
recent OECD and EEC guidelines.
Batch 200502771-6M of Unoxol™ 3,4 Dialdehyde was a clear to very
pale-yellow liquid. The test substance was dissolved
In the dose range finding test, Unoxol™ 3,4 Dialdehyde was tested up to
concentrations of 5000 μg/plate in the absence and
presence of S9-mix in the strains TA100 and WP2uvrA. Unoxol™ 3,4
Dialdehyde did not precipitate on the plates at this
dose level. Cytotoxicity was evidenced by a complete lack of any
microcolony background lawn at the dose levels of 3330
and 5000 μg/plate in both tester strains. Results for this dose range
finding test were reported as part of the first experiment of
the mutation assay.
Based on the results of the dose range finding test, Unoxol™ 3,4
Dialdehyde was tested in the first mutation assay at a
concentration range of 33 to 3330 μg/plate in the absence and presence
of 5% (v/v) S9-mix in tester strains TA1535, TA1537
and TA98. Cytotoxicity was evidenced by a complete lack of any
microcolony background lawn in all three tester strains.
In an independent repeat of the assay with additional parameters,
Unoxol™ 3,4 Dialdehyde was tested at a concentration
range of 10 to 2000 μg/plate in the absence S9-mix and at 10 to 3330
μg/plate in the presence of 10% (v/v) S9-mix in tester
strains TA1535, TA1537, TA98, TA100 and WP2uvrA. Cytotoxicity was
observed in all tester strains, except in tester strain
WP2uvrA in the absence of S9-mix.
Unoxol™ 3,4 Dialdehyde did not induce a significant dose-related
increase in the number of revertant (His+) colonies in each
of the four tester strains (TA1535, TA1537, TA98 and TA100) and in the
number of revertant (Trp+) colonies in tester strain
WP2uvrA both in the absence and presence of S9-metabolic activation.
These results were confirmed in an independently
In this study, the negative and strain-specific positive control values
were within our laboratory historical control data ranges
indicating that the test conditions were adequate and that the metabolic
activation system functioned properly.
Based on the results of this study it is concluded that Unoxol™ 3,4
Dialdehyde is not mutagenic in the Salmonella
typhimurium reverse mutation assay and in the Escherichia coli reverse
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