Vinyl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, near guideline methodology, published in peer reviewed literature, adequate for assessment

Data source

Reference

Reference Type:

publication

Title:

Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals

Author:

McCann J, Choi E, Yamaski E & Ames BN

Year:

1975

Bibliographic source:

Proc. Nat. Acad. Sci USA, Vol 72, No. 12, pp5135-5139

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Vinyl acetate

EC Number:

203-545-4

EC Name:

Vinyl acetate

Cas Number:

108-05-4

Molecular formula:

C4H6O2

IUPAC Name:

ethenyl acetate

Details on test material:

- Name of test material (as cited in study report): vinyl acetate
- Source: Aldrich
- Purity: highest available

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Aroclor induced rat liver S9 mix

Test concentrations with justification for top dose:

No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation);
- Homogenates of rat (or human) liver (S9 mix) were added directly to petri plates to evaluate the need for metabolic activation.

Method described in detail in Ames B N , McCann J & Yamasaki E (1975). Mutation Research.

Evaluation criteria:

Number of revertants per plate (histidine revertants on a petri plate/number of micrograms tested).

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

not applicable

Untreated negative controls validity:

not applicable

Positive controls validity:

not applicable

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

Vinyl acetate was negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation.

Executive summary:

Approximately 300 carcinogens and non-carcinogens of a variety of chemical types were tested for mutagenicity in the Salmonella/microsome test. Bacteria were used as sensitive indicators for DNA damage and mammalian liver extracts for metabolic conversion of carcinogens to their active metabolic forms. Vinyl acetate was shown to be negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation.