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EC number: 202-486-1
CAS number: 96-18-4
Table 4: Mean measured algal cell densities during the test periodA
Mean cell density x 104(cells/mL)
On day 0 the cell density of the preculture was determined. Based on
this result a quantity 0.265 mL of the preculture was added to all serum
bottles resulting in an initial concentration of 1.0 x 10 exp 4 cells/mL.
The measured algal cell densities given in table 5 are mean cell
densities of all serum bottles. The data show that the cell density
increased 134 — 174 times in the controls, which meets the validity
criterion of a minimal 16 times increase.
Table 5: Mean biomass integral and biomass integral inhibition (%) on
Biomass integral x 104
Biomass integral inhibitionon day 3 (%)
The cell densities (unrounded data) of each serum bottle were used to
calculate the area under the growth curve (biomass integral). The mean
biomass Integral and the biomass Integral inhibition is given in Table 4
for each test concentration.
Table 6: Mean growth rate inhibition (%) at various measured
Growth rate inhibition (%)
Day 1 -2
Day 2 - 3
Day 0 - 3
The toxicity of 1,2,3-trichloropropane to algae (Pseudokirchnerella
subcapitata) was tested according to OECD GLP Principles of Good
Laboratory Practice and Compliance Monitoring No. 1, ENV/MC/CHEM(98)17
of 21 January 1998 and according to OECD Guideline 201 (OECD 1984) with
an adaptation of the algal medium (extra amount of NaHCO3)
to enable the use of a closed system. 1,2,3-Trichloropropane is volatile
and for this reason algae were exposed for three days using closed
system flasks containing 130 mL test solution. 1,2,3-Trichloropropane
was tested at nominal test concentrations of 0, 10, 20, 40, 80 and 160
mg/L in algal medium.
To study the exposure of the algae to 1,2,3-trichloropropane, samples of
the test solutions were taken at the start, day 1, day 2 and on day 3 of
the test and analyzed with Gas Chromatography. The difference between
the nominal and the mean measured concentrations was larger than 20 %
and for this reason the biological endpoints of the test are based on
the average of the measured concentration being 0, 7.3, 12.8, 24.8, 48.3
and 101 mg/L.
The cell density increased 134 — 174 times in the controls, which meets
the validity criterion of a minimal 16 times increase. The cell density
measurements were used to calculate the biomass integral and growth rate
for each serum bottle. Using linear interpolation, with mean measured
concentrations on a logarithmic scale (base = 10), the EC50 0-72h for
biomass integral and growth rate were 49.6 and > 101 mg/L, respectively.
The No Observed Effect Concentration (NOEC), based on biomass integral
at test termination, was determined with Williams' Test (Williams 1972).
Based on mean measured concentrations this revealed a NOEC of 12.8 mg/L.
The acute toxic effects of 1,2,3 TCP to algae are characterized by a EC50 of 49.6 mg/L and a NOEC of 12.8.
Data exist from three algal species (Chlorella vulgaris,
Chlamydomonas angulosa, Pseudokirchnerella subcapitata). Hutchinson
et al (1980) tested the former two species in 3 h exposure experiment
were inhibition of metabolic rate was measured as reduction of carbon
dioxide uptake. The determined EC50
values of 170 and 112 mg/L respectively were not regarded for
assessment as the method is considered not acceptable.
Reliable figures are available form de Groot (2002) on green alga (Pseudokirchnerella
subcapitata). The study is valid and conclusive and a 72-h ErC50
value of 101 mg/L (i.e. related to the endpoint growth rate),
which is the relevant value with regard to GHS/CLP, was obtained. This
study (de Groot 2002) finds an acute 72-h NOEC of 12.8 mg/L.
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