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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP study, but limited documentation

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1991
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report Date:
1985

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Remarks:
but adjustment to the properties of the test material (see Preparation of test solution for soluble substances)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Source: Nippon Steel Chemical Comp.
- Name of test material (as cited in study report): Pitch (coal tar)
- Substance type: organic
- Physical state: solid
- Analytical purity: not applicable
- Volatiles 2.4 % at 270 and 15.6 % at 350 °C [Note: The fraction of volatiles was higher than in EU pitch]
- Softening point 36.5 °C (Yoshida, 1985, Annex 1, p. 17).


- Composition of test material, percentage of components (GC-analysis):

Content [mg/kg pitch] (Yoshida 1985, p. 18)
=================================
Naphthalene ca. 22500
2-Methylnaphthalene ca. 3700
1-Methylnaphthalene ca. 1600
Biphenyl ca. 1100
Acenaphthene ca. 9400
Fluorene ca. 1100
Phenanthrene
Anthracene(Phen.+ Anthr.) ca. 70600
Fluoranthene ca. 44200
Pyrene ca. 39800
Benz(a)anthracene no data
Chrysene ca. 42900
Benzo(b)fluoranthene no data
Benzo(k)fluoranthene no data
Benzo(a)pyrene no data
Indeno[1,2,3cd]pyren no data
Dibenzo[a,h]anthracene no data
Benzo[ghi]perylene no data
=================================

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Direct dosing method A: Each test solution prepared separately without separation of undissolved matter (settling for 7 h);
-Direct dosing method B: Each test solution prepared separately with separation of the supernatant from not dissolved matter
but without further filtration.Stirring time was 24 h for coal-tar pitch at 25 +-2 °C.

Coal tar pitch was spread over glass plates at 50 mg/cm2, and a corresponding number of plates was used for a 24-h extraction in test medium.

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Red killifish
- Source: Nakashima Fish Farm, Arao city
- Age at study initiation (mean and range, SD): no data
- Length at study initiation: mean 3.2 cm
- Weight at study initiation: mean 0.25 g

ACCLIMATION
- Acclimation period: 14 d
- Type and amount of food: no data
- Feeding frequency: no data


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
water renewal each 24 h
Post exposure observation period:
none

Test conditions

Hardness:
no data
Test temperature:
25 ± 2°C
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Nominal: no data, >= 5 loadings spaced at a factor of 1.8
Analysis: The water-soluble fraction was about 0.13 - 0.07 % of the nominal amount and ranged from ca. 0.630 - 0.670 mg/L 
(total of 11 substances, incl. naphthalene) at nominal 500 and 1000 mg/l, respectively (Yoshida, 1985, Tab. A17, p. 38).   
----------------------------
After 24 h, ca. 50 % of the initial concentration was recovered from 1000 mg/l pitch (Yoshida 1985, p. 11 and Tab. A17, p. 38
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open, covered with aluminium foil
- Material, size, headspace, fill volume: glass vessel (29.5 x 15 cm in diameter), test volume = 5 litres
- Aeration: probably no ("sufficiently aerated" medium was taken)
- Renewal rate of test solution (frequency/flow rate): 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: estimated 5 L/10 x0.25 g = approx. 2 L/g fish


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light
- Light intensity: no data


TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Range finding study
- Test concentrations: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 - < 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
: 10/10 (100 %) animals died at 1000 mg/L
Duration:
96 h
Dose descriptor:
LL0
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
: 1/10 (10 %) and 0/10 animals died (relating to Method A and B, resp.) [(see above Details on test solution)]
Duration:
96 h
Dose descriptor:
LL100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Exact LL50 had not be determined
Reported statistics and error estimates:
not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
Criteria for poorly soluble substances fulfilled