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EC number: 212-110-8
CAS number: 763-32-6
It can be stated that under the experimental conditions reported the
test item did not induce gene mutations at the HPRT locus in V79 cells.
Therefore, Isoprenol is considered to be non-mutagenic in this HPRT
According to the results of the present study, the test substance
3-Methylbuten-3-ol-1 is not mutagenic in the Ames test under the
experimental conditions chosen here.
The study was performed to investigate the potential of Isoprenol to
induce gene mutations at the HPRT locus in V79 cells of the Chinese
The assay was performed in two independent experiments, using two
parallel cultures each. The first main experiment was performed with and
without liver microsomal activation and a
treatment period of 4 hours. The second experiment was performed with a
treatment time of 4 hours with and 24 hours without metabolic activation.
The highest concentration of the test item (880 μg/mL) was equal to a
molar concentration of about 10 mM.
The tested concentrations are described in Table 2 (page 19). The
evaluated experimental points and the results are summarised in Table 1
No substantial and reproducible dose dependent increase of the mutation
frequency was observed in both main experiments.
Appropriate reference mutagens, used as positive controls, induced a
distinct increase in mutant colonies and thus, showed the sensitivity of
the test item and the activity of the metabolic activation system.
It can be stated that under the experimental conditions reported, the
test item did not induce micronuclei as determined by the micronucleus
test with bone marrow
cells of the mouse. Therefore, Isoprenol is considered to be
non-mutagenic in this micronucleus assay.
This study was performed to investigate the potential of Isoprenol to
induce micronuclei in polychromatic erythrocytes (PCE) in the bone
marrow of the mouse.
The test item was formulated in corn oil, which was also used as vehicle
control. The volume administered orally was 10 mLlkg b.w.. 24 hand 48 h
after a single administration of the test item the bone marrow cells
were collected for micronuclei analysis.
Seven males per test group (except the vehicle and positive control
groups with 5 males each) were evaluated for the occurrence of
micronuclei. Per animal 2000 polychromatic erythrocytes (PCEs) were
scored for micronuclei.
To describe a cytotoxic effect due to the treatment with the test item
the ratio between polychromatic and normochromatic erythrocytes was
determined in the same sample and reported as the number of PCEs per
After treatment with the test item the number of PCEs was not
substantially decreased as compared to the mean value of PCEs of the
vehicle control thus indicating that Isoprenol
did not exert any cytotoxic effects in the bone marrow. In comparison to
the corresponding vehicle controls there was no biologically relevant or
statistically significant enhancement in the frequency of the detected
micronuclei at any preparation interval after administration of the test
item and with any dose level used. Cyclophosphamide as positive control
showed a substantial increase of induced micronucleus frequency.
The available information is conclusive but not sufficient for
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