Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, non-GLP. However, no details on the test substance characteristics given.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988
Reference Type:
other: recalculation
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German Standard Guideline DIN  38412 L9 
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
no further details on the test substance characteristics given

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain: Scenedesmus subspicatus SAG 86.81
- Source (laboratory, culture collection): Sammlung für Algenzellkulturen, University of Göttingen)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20 °C
Nominal and measured concentrations:
Nominal test concentration (mg/l):
31.25, 62.5, 125, 250, 500, control
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 ml glass tubes with flat bottom
- Fill volume: 10 ml

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
172.05 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
279.67 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
454.61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Cell densities after x hours of exposure in fluorescence units

Nominal test  / Exposure time [h]
concentration /
(mg/l)        /  0 / 24 /  48 /  72 /   96 / 
Control       / 33 / 155 / 808 / 3279 /  5477 /
31.25         / 32 / 150 / 830 / 3127 /  5878 /
62.5          / 33 / 144 / 791 / 2977 /  5552 /
125           / 33 / 117 / 592 / 2553 /  5142 /
250           / 32 /  60 / 187 /  579 /  1379 /
500           / 33 /  33 /  35 /   41 /    52 /

Growth rate and yield values and % inhibition of growth rate and yield  after 72 h

             growth rate r / % inhibition / yield / % inhibition
Control          / 1.537 /  -           / 3245.940 /  -
31.25          / 1.524 /  0.846 / 3094.580 /  4.663
62.5          / 1.500 /  2.407 / 2944.520 /  9.286
125          / 1.453 /  5.465 / 2520.440 / 22.351
250          / 0.960 / 37.541 /  547.300 / 83.139
500          / 0.077 / 94.990 /    8.540 / 99.737


EC-values after 72 hours:
growth rate
ErC10: 172.05 mg/l
ErC50: 279.67 mg/l
NOEC: 62.5 mg/l
LOEC: 125 mg/l

Yield
EyC10: 98.55 mg/l
EyC50: 169.04 mg/l
NOEC: 62.5 mg/l
LOEC: 125 mg/l

Applicant's summary and conclusion