3-methylbut-3-en-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. Comparable to OECD 202.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EG guideline 79/831/EWG, Annex V,  part C

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-3-en-1-ol

EC Number:

212-110-8

EC Name:

3-methylbut-3-en-1-ol

Cas Number:

763-32-6

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-3-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
- Lot/batch No.: 31-0342
- Analytical purity: 99%;
water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 500 mg/l) in  culture medium was prepared. Then, this test concentration was serially  diluted with culture medium to obtain the desired test concentrations. 

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de  Recherche Chimique Appliquee, France and cultured in the laboratories of  BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 +/- 0.5 mmol/l

Test temperature:

19 - 21 °C

pH:

8.0 - 8.2

Dissolved oxygen:

8.3 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
31.25,
62.5,
125,
250,
500,
control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes (nominal 20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Mixture of dechlorinated tap water and  distilled water, acidified with H2SO4 (p.a.)
- Molar ratio Na:K: about 10 : 1
- Alkalinity up to pH 4.3: 0.8 +/- 1.0 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 550 - 650 microSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at  400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Inhibition of swimming ability of daphnids after 3, 6, 24 and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Probit analysis

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) 0 / 3 / 6 / 24 / 48
Control /   0 / 0 / 0 / 0 / 0
31.25   /   0 / 0 / 0 / 0 / 0
62.5    /   0 / 0 / 0 / 0 / 0
125     /   0 / 0 / 0 / 0 / 0
250     /   0 / 0 / 0 / 0 / 0
500     /   0 / 0 / 0 / 0 / 10

24 hour ECx values:
EC0: >= 500 mg/l
EC50: > 500 mg/l
EC100: > 500 mg/l

All biological results refer to nominal test concentrations.