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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study. Comparable to OECD 202.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EG guideline 79/831/EWG, Annex V,  part C
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylbut-3-en-1-ol
EC Number:
212-110-8
EC Name:
3-methylbut-3-en-1-ol
Cas Number:
763-32-6
Molecular formula:
C5H10O
IUPAC Name:
3-methylbut-3-en-1-ol
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
- Lot/batch No.: 31-0342
- Analytical purity: 99%;
water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 500 mg/l) in  culture medium was prepared. Then, this test concentration was serially  diluted with culture medium to obtain the desired test concentrations. 

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de  Recherche Chimique Appliquee, France and cultured in the laboratories of  BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.70 +/- 0.5 mmol/l
Test temperature:
19 - 21 °C
pH:
8.0 - 8.2
Dissolved oxygen:
8.3 - 9.1 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
31.25,
62.5,
125,
250,
500,
control
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes (nominal 20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Mixture of dechlorinated tap water and  distilled water, acidified with H2SO4 (p.a.)
- Molar ratio Na:K: about 10 : 1
- Alkalinity up to pH 4.3: 0.8 +/- 1.0 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 550 - 650 microSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at  400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Inhibition of swimming ability of daphnids after 3, 6, 24 and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Probit analysis

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) 0 / 3 / 6 / 24 / 48
Control /   0 / 0 / 0 / 0 / 0
31.25   /   0 / 0 / 0 / 0 / 0
62.5    /   0 / 0 / 0 / 0 / 0
125     /   0 / 0 / 0 / 0 / 0
250     /   0 / 0 / 0 / 0 / 0
500     /   0 / 0 / 0 / 0 / 10

24 hour ECx values:
EC0: >= 500 mg/l
EC50: > 500 mg/l
EC100: > 500 mg/l

All biological results refer to nominal test concentrations.

Applicant's summary and conclusion