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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets national standard methods (DIN).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German standard DIN 38412, part 8
Principles of method if other than guideline:
Growth-inhibition test according to Bringmann-Kühn.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
no further details on the test substance characteristics given

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida, strain DSM 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
24 +/- 1 °C
Nominal and measured concentrations:
Nominal Test concentrations (mg/l): 312.5, 625, 1250, 2500, 5000, 7500, 10000, control
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 300 ml)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium (DIN 38412)

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
1 350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
1 870 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
2 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

EC10 = 1349.6 mg/l
EC50 = 1874.2 mg/l
EC90 = 2398.8 mg/l

pH values (range over all concentrations):
test preparation, uninoculated, at 0 h: 6.7 - 7.2; blank: 7.1
test preparation, uninoculated, at 17 h: 6.7 - 7.1; blank: 7.1
parallel no. 1, at 17 h: 5.6 - 6.7; blank: 5.6

Applicant's summary and conclusion