3-methylbut-3-en-1-ol

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets national standard methods (DIN).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, part 8

Principles of method if other than guideline:

Growth-inhibition test according to Bringmann-Kühn.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-3-en-1-ol

EC Number:

212-110-8

EC Name:

3-methylbut-3-en-1-ol

Cas Number:

763-32-6

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-3-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
no further details on the test substance characteristics given

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Pseudomonas putida, strain DSM 50026

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Test temperature:

24 +/- 1 °C

Nominal and measured concentrations:

Nominal Test concentrations (mg/l): 312.5, 625, 1250, 2500, 5000, 7500, 10000, control

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 300 ml)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium (DIN 38412)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

1 350 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

1 870 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 3

Duration:

17 h

Dose descriptor:

other: EC90

Effect conc.:

2 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Any other information on results incl. tables

EC10 = 1349.6 mg/l
EC50 = 1874.2 mg/l
EC90 = 2398.8 mg/l

pH values (range over all concentrations):
test preparation, uninoculated, at 0 h: 6.7 - 7.2; blank: 7.1
test preparation, uninoculated, at 17 h: 6.7 - 7.1; blank: 7.1
parallel no. 1, at 17 h: 5.6 - 6.7; blank: 5.6