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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study. Comparable to OECD 203.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German standard, DIN 38 412 L15.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylbut-3-en-1-ol
EC Number:
212-110-8
EC Name:
3-methylbut-3-en-1-ol
Cas Number:
763-32-6
Molecular formula:
C5H10O
IUPAC Name:
3-methylbut-3-en-1-ol
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
- Lot/batch No.: 31-0342
- Analytical purity: 99%;
water solubility: 90 g/l, according to sponsor.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test substance to the test  media without any pre-treatment. Then, the fish were placed into the test  aquaria.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany  
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range 5.7 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.9 g (range 1.5 - 4.4 g)
- Feeding during test: 1 day before and during exposure
- Corpulence factor of the test fish batch: 0.95
- Housing and adaption  Culture conditions: oil-free aerated and charcoal filtered tap water,  flow-through system Water temperature: 20 -  21 °C   Duration of housing: about 2 months  Mortality during the last 2 weeks of housing: 2.3 % , Medical treatment: Twice with 0.05 mg/l malachite green chloride, once  with 10 mg/l tetracycline hydrochloride. 

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum. 
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21 °C 
pH:
7.3 - 7.6 
Dissolved oxygen:
6.9 - 8.7 mg/l
Nominal and measured concentrations:
Nominal test concentrations: 46.4, 100, 215, 464, 1000 mg/l (based on the  results of a range-finding test). In addition, a control containing test  water only was tested in parallel. 
Details on test conditions:
TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm) 
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.4 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II,  1982; prepared from fully demineralised tap water (conductivity: max 10  micro MHO) by adding: 294.0 mg/l CaCl2 * 2 H2O, 123.3 mg/l MgSO4 * 7 H2O,  63.0 mg/l NaHCO3, 5.5 mg/l KCl 
- Ratio Na/K ions: 10 : 1
- Acid capacity: 0.8 mmol/l
- Ca/mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours day-night regime 


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
460 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
460 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
681.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
48 h-LC50 of the positive control Chloroacetamide: about 32 mg/l  (corresponds to normal sensitivity).
Reported statistics and error estimates:
The EC50 value was calculated as the geometrical mean of the LC0 (464.  mg/l) and the LC100 (1000 mg/l) value.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to 3-methylbuten-3-ol-1, mortalities were as  follows:

Nominal test       Cumulative mortalitiy (%) after x hours
concentration       1  /  4  / 24  / 48  / 72  / 96
(mg/l)
Control             0  /  0  /  0  /  0  /  0  /  0
46.4                0  /  0  /  0  /  0  /  0  /  0
100.0               0  /  0  /  0  /  0  /  0  /  0
215.0               0  /  0  /  0  /  0  /  0  /  0
464.0               0  /  0  /  0  /  0  /  0  /  0
1000.0              1  /  2  / 10  / 10  / 10  / 10

Symptoms of intoxication:

Nominal test       Symptoms of intoxication after x hours
concentration      1 / 4 /24 / 48 / 72 / 96
(mg/l)
Control             - / - / - / - / - / -
46.4                - / - / - / - / - / -
100.0               - / - / - / - / - / -   
215.0               - / - / - / - / - / -
464.0               - / - / - / - / - / -
1000.0              T / T / + / + / + / +

Definitions of Symptoms:
-: No symptoms
+: All fish dead
T: Tumbling

All results refer to nominal test concentrations of 3-methylbuten-3-ol-1.
No analytical dose-verification of the test item was carried out.

Behavior of the test item during the test:
No remarkable observations.

Applicant's summary and conclusion