Ethylenediamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

ca. 1948

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1948

Report date:

1948

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Concentrated and 10% water solution was applied to the clipped male rabbit trunk under Vinylite sheet

GLP compliance:

no

Remarks:

did not exist at the time

Test type:

other: Comparable to traditional method (guideline did not exist at that time)

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethylenediamine

EC Number:

203-468-6

EC Name:

Ethylenediamine

Cas Number:

107-15-3

Molecular formula:

C2H8N2

IUPAC Name:

ethane-1,2-diamine

Details on test material:

Concentration 76% ethylenediamine

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 h

Doses:

0.5 - 0.63 - 0.795 - 1 mg/kg bw of concentrated sample (76%)
10% solution: 1.20 - 1.52 mg/kg bw

No. of animals per sex per dose:

10 males

Control animals:

no

Statistics:

Not required for this type of study

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

560 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

approximate LD50

Effect level:

ca. 1 520 mg/kg bw

Remarks on result:

other: as 10% solution

Mortality:

Yes

Clinical signs:

other:

Gross pathology:

Necrosis of the skin, congestion of the lungs and damage to the abdominal organs was noted on autopsy.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The LD50 value in an acute dermal toxicity test on male rabbits was 560 mg/kg bw.

Executive summary:

Rabbits were exposed to concentrated ethylene diamine (76%) and a 10% dilution in water under occlusive coverage for 24 h. With the concentrated substance, severe skin damage occurred. Congestion of the lungs and damage to the abdominal organs was noted on autopsy. LD50 was 560 mg/kg bw. For the diluted samples LD50 was approximately 1520 mg/kg bw.