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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Deviations:
yes
Remarks:
Ammoniumchloride was ommitted from the medium to prevent nitrification.
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Activated sludge, from an activated sludge plant, was used as inoculum. Secondary activated sludge was obtained from the rzwi Nieuwgraaf in Duiven (1989.02.27). The rzwi Nieuwgraaf is a domestic sewage treatment plant. The sludge was pre-conditioned to reduce the endogenous respiration rates by aeration for a period of three days. The sludge concentration was 2 mg/dm3.
Ammoniumchloride was omitted from the medium to improve the accuracy of the quantication of the biodegradability.
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
acetic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
10
Sampling time:
5 d
Parameter:
% degradation (O2 consumption)
Value:
88
Sampling time:
15 d
Parameter:
% degradation (O2 consumption)
Value:
95
Sampling time:
28 d

 Time (days) oxygen consumption (mg/L)     Biodegradation (%)    
   Test Acetate  Test  Acetate 
 0 0.0  0.0 
5 0.7 3.0 10 75
15 6.1 3.7 88 93
28 6.6 3.8 95 95
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Ethylenediamine is degraded 95% in 28 days in the Closed Bottle Test and can be considered readily biodegradable.
Executive summary:

A study was conducted to determine the ready biodegradability of the test substance in water via measurement of O2 consumption according to EU Method C.4-E, in compliance with GLP. Activated sludge from an activated sludge plant treating predominantly domestic sewage, was used as inoculum. The sludge was pre-conditioned to reduce the endogenous respiration rate by aeration for a period of 3 d. The sludge concentration was 2 mg/dm3. Sodium acetate was used as the reference substance. The test substance degraded 88% in 15 d and at 95% in 28 d. The validity criteria of the study was fulfilled. Under the study conditions, the substance was readily biodegradable (van Ginkel, 1989).

Description of key information

Ethylenediamine is readily biodegradable in screening tests.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A study was conducted to determine the ready biodegradability of the test substance in water via measurement of O2 consumption according to EU Method C.4-E, in compliance with GLP. Activated sludge from an activated sludge plant treating predominantly domestic sewage, was used as inoculum. The sludge was pre-conditioned to reduce the endogenous respiration rate by aeration for a period of 3 d. The sludge concentration was 2 mg/dm3. Sodium acetate was used as the reference substance. The test substance degraded 88% in 15 d and at 95% in 28 d. The validity criteria of the study was fulfilled. Under the study conditions, the substance was readily biodegradable (van Ginkel, 1989).